LOCUS       sequence-166583-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  csd2, construct: 121-308, pET21a(+) no-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   An DR, Im HN, Jang JY, Kim HS, Kim J, Yoon HJ, Hesek D, Lee M, 
            Mobashery S, Kim SJ, Suh SW
  TITLE     Structural Basis of the Heterodimer Formation between Cell 
            Shape-Determining Proteins Csd1 and Csd2 from Helicobacter pylori.
  JOURNAL   PLoS One. 2016 Oct 6;11(10):e0164243. doi: 
            10.1371/journal.pone.0164243. eCollection 2016.
  PUBMED    27711177
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-10-6"; volume: "11(10):e0164243. doi"; pages: " 
            10.1371/journal.pone.0164243. eCollection 2016"
COMMENT     Sequence Label: csd2_121-308/pET21a(+)
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             636..658
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    673..697
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(697..716)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(698..716)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(800..819)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtt
       61 actggcttat gagtgcgtct ttatccacat gctcttggag ctggacaatg tcttctaaaa
      121 cccaaaacaa attcttccat tcaataaatt tattttcttc taaagcgctt tgaaaatgat
      181 ccaaatccca tgccagaaat ttttgcgcgt ctaaaatttt acccaaaaag cgcacttcat
      241 aatgcaattt ttcgccgccg ctattaccgc tcttcccgct atagccaatc aactgccctt
      301 tttggatgaa gcttttgggc tgcacattga catgatctaa gtgcgtgtaa atggagctaa
      361 aaccaaacgc atgttcaatg cgcaccaagt tcccataccc cacattagaa ttggtcttca
      421 caaaatccac aatcccatca gcgctcgcat aaacaggcgt gtttaatggc gcgataaaat
      481 caatcccgga ttcaacgccc ttaatttttt taatggggtg gttcctttct ttggtgggtt
      541 tgatagcgct ataagttttt atgggcatgc cgttaggaat gagcatgagc gctaaatgtt
      601 tttgagccag attcaaattg tctaacatat gtatatctcc ttcttaaagt taaacaaaat
      661 tatttctaga ggggaattgt tatccgctca caattcccct atagtgagtc gtattaattt
      721 cgcgggatcg agatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
      781 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
      841 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggc
//
