LOCUS       sequence-166584-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Drosophila P-element transgenesis vector for cloning rescue 
            transgenes. Cloning site is flanked by FRT sequences and contains 
            constitutive RFP reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Zhou Q, Neal SJ, Pignoni F
  TITLE     Mutant Analysis by Rescue Gene Excision: New tools for mosaic 
            studies in Drosophila.
  JOURNAL   Genesis. 2016 Oct 3. doi: 10.1002/dvg.22984.
  PUBMED    27696669
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2016 Oct 3. doi: 10.1002/dvg.22984."
COMMENT     Sequence Label: FoRC.PRa
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     195..213
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    complement(387..434)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    complement(682..729)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     879..896
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     880..896
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tttctgtgga cagagaagga ggcaaacagc gctgactttg agtggaatgt cattttgagt
       61 gagaggtaat cgaaagaacc tggtacatca aatacccttg gatcgaagta aatttaaaac
      121 tgatcagata agttcaatga tatccagtgc agtaaaaaaa aaaaatgttt tttttatcta
      181 ctttccgcaa aaatgggttt tattaactta catacatact agaattcgtt aacagatctg
      241 cggccgcgaa ttcactagtg attaagctag cgaattcgtt tgtggaagcg gtattcgcaa
      301 tattttagta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga
      361 gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc
      421 ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg
      481 cgcacataca gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata
      541 tacatgagaa gaacggcata gtgcgtgaat tcgtttgtgg aagcggtatt cgcaatattt
      601 tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtct tcagagcgct
      661 tttggttttc aaaagcgctc tgaagttcct atactttcta gagaatagga acttcggaat
      721 aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac
      781 atacagctca ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat atatatacat
      841 gagaagaacg gcatagtgcg tcctgcagga ggccggcctg taaaacgacg gccagtctta
      901 agctcgggcc ccaaataa
//
