LOCUS       sequence-16659-e        1338 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L2684
FEATURES             Location/Qualifiers
     source          1..1338
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             557..577
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(620..648)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gnggctgcgg tcggctctag aggatcacca aaaacggaac gttgagctgg acggaaatag
       61 tggtanagtg acatgattat agtttgaaga tctctaattt cacaattaga gcaaatgttg
      121 ttcggtattt attttcaacg gtatttatac tattttccac ctttttctag aacattcgag
      181 ctgcttgttg caaaaggagg gcgactcaca ttcggtacat ggaaaagtag tgtacacaat
      241 aaagagaccc agatacattt tccgtctgcg tctctttgca cccaccggga gtattttcaa
      301 acgaatgcat ctaggacctt ctagaacatt ctgtaaggct gcagaatgcg ggtatataag
      361 gaaagcgggc tcagaggaag ccaacacgct ttgttctagt gcatctaaaa aacttcgaaa
      421 atcctcatcg gatccccggg attggccaaa ggacccaaag gtatgtttcg aatgatacta
      481 acataacata gaacattttc aggaggaccc ttggctagag gatccccggg taccgagctc
      541 agaaaaaatg actgctccaa agaagaagcg taaggtaccg gtagaaaaaa tgagtaaagg
      601 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      661 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      721 taaatttatt tgcactactg gaaaactacc tgttccatgg gtaagtttaa acatatatat
      781 actaactaac cctgattatt taaattttca gccaacactt gtcactactt tctgttatgg
      841 tgttcaatgc ttctcgagat acccagatca tatgaaacgg catgactttt tcaagagtgc
      901 cntgcccgaa ggttatgtac aggaaaagaa ctatattttt caagatgacg ggaactacca
      961 gacacgtang tttaaacagt tcggtactaa ctaaccatac atatttaatt ttcnggtgct
     1021 gaagtcaagt ttgaaggtga tnccctgtta aaaaatcnag taaangnatt gatttnaaga
     1081 aagggaacnt ctggaccant ggaaacacta tactccccca ngatcctctg gnnaaacaaa
     1141 gagggatcaa gtggagttaa ctgatttcca cacaacgata attncaactc catnaaccnc
     1201 tggnaggggt cctnnnactt cnaaaccntg ggnggcgttt nnaaactncg ccnaatgctt
     1261 aaacaaaaaa cngcctnntt accngganng ggaaaaaant tanccgcccc cccnctngga
     1321 aaggcncaaa aaattaaa
//
