LOCUS       sequence-166595-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express copGFP reporter for HDR-induced knock-in.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   He X, Tan C, Wang F, Wang Y, Zhou R, Cui D, You W, Zhao H, Ren J, 
            Feng B
  TITLE     Knock-in of large reporter genes in human cells via 
            CRISPR/Cas9-induced homology-dependent and independent DNA repair.
  JOURNAL   Nucleic Acids Res. 2016 May 19;44(9):e85. doi: 10.1093/nar/gkw064. 
            Epub 2016 Feb 4.
  PUBMED    26850641
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-05-19"; volume: "44(9):e85. doi"; pages: " 
            10.1093/nar/gkw064. Epub 2016 Feb 4"
COMMENT     Sequence Label: 2a-copGFP(+HA)
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(93..149)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ccgcccacca gctcgaactc cacgccgttc agggtgccgg tgatgcggca ctcgatctcc
       61 atggcgggca ggccgctctc gtcgctctcc attggcccgg gattctcttc gacatcccct
      121 gcttgtttca acagggagaa gttagtggca cgcgtctcct tggaggccat gtgggccatg
      181 aggtccacca ccctgttgct gtagccaaat tcgttgtcat acctagaaga tgaaaagagt
      241 tgtcagggcc ctttttctga gccagccacc agagggcgcc agaccctgca ctttttaaga
      301 gccagtctct ggccccagcc acataccagg aaatgagctt gacaaagtgg tcgttgaggg
      361 caatgccagc cccagcgtca aaggtggagg agtgggtgtc gctgttgaag tcagaggaga
      421 ccacctggtg ctcagtgtag cccaggatgc ccttgagggg gccctccgac gcctgcttca
      481 ccaccttctt gatgtcatca tatttggcag gtttttctag acggcaggtc aggtccacca
      541 ctgacacgtt ggcagtgggg acacggaagg ccatgccagt gagcttcccg ttcagctcag
      601 ggatgacctt gcccacagcc ttggcagcgc cagtagaggc agggatgatg ttctggagag
      661 ccccgcggcc atcacgccac agtttcccgg aggggccatc cacagtcttc tgggtggcag
      721 tgatggcatg gactgtggtc tgcaaaagga gtgaggccct gcagcgtact ccccacatca
      781 cccctctacc tccctcccca ccttgaaagg aaattatggg
//
