LOCUS       sequence-16660-e        1335 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L2685
FEATURES             Location/Qualifiers
     source          1..1335
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             555..575
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(618..646)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggctgcggtc gactctagag gatcaccaaa aacggaacgt tgagctggac ggaaatagtg
       61 gtaaagtgac atgattatag tttgaagatc tctaatttca caattagagc aaatgttgtt
      121 cggtatttat tttcaacggt atttatacta ttttccacct ttttctagaa cattcgagct
      181 gcttgttgca aaaggagggc gactcacatt cggtacatgg aaaagtagtg tacacaataa
      241 agagacccag atacattttc cgtctgcgtc tctttgcacc caccgggagt attttcaaac
      301 gaatgcatct aggaccttct agaacattct gtaaggctgc agaatgcggg tatataagga
      361 aagcgggctc agaggaagcc aacacgcttt gttctagtgc atctaaaaaa cttcgaaaat
      421 cctcatcgga tccccgggat tggccaaagg acccaaaggt atgtttcgaa tgatactaac
      481 ataacataga acattttcag gaggaccctt ggctagagga tccccgggta ccgagctcag
      541 aaaaaatgac tgctccaaag aagaagcgta aggtaccggt agaaaaaatg agtaaaggag
      601 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      661 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      721 aatttatttg cactactgga aaactacctg ttccatgggt aagtttaaac atatatatac
      781 taactaaccc tgattattta aattttcagc caacacttgt cactactttc tgttatggtg
      841 ttcaatgctt ctcgagatac ccagatcata tgaaacggca tgactttttc aagagtgccn
      901 tgcccgaagg tnatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
      961 cacgtangtt taaacagttc ggtactaact ancntacata tttaantttc nggtgctgaa
     1021 gtcaagttgg angtgatccc tngttataga tcnagttaaa ggtatgattt naagaaangg
     1081 aacttctgga ccaatnggaa acactatact ccncatgtna ctctggcnan aacaaaaang
     1141 anncaagtgn agttaaccga tttccaccac aacngattat ttcnactcaa taacccntgg
     1201 aggnggtcct gcaacnttcc aaaancctgg ggggcgtctt naaaanattt gnccnantgc
     1261 nttnaaacna aaaaaangcc tnttttaccn ggaacngggg aaaaancngg ggaanaacnc
     1321 nccngttcct tcctt
//
