LOCUS       sequence-166602-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single Integration Vector TRP - Cloning vector for single 
            integration into the TRP1 locus of S.cerevisiae containing two MCS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: SIVt
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        345..363
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    376..483
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     392..408
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     393..409
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(443..459)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(443..459)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(491..510)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(492..510)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(511..544)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttcgaa ttaattaact agcaagaatc gggtcattgt agcgtatgcg
      121 cctgtgaaca ttctcttcaa caagtttgat tccattgcgg tgaaatggta aaagtcaaac
      181 ccctgcgatg tatattttcc tgtacaatca atcaaaaagc caaatgattt agcattatct
      241 ttacatcttg ttattttaca gattttatgt ttagatcatt tatgcttgct tttcaaaagg
      301 cttgcaggca agtgcacaaa caatacttaa ataaatactc aggaaattaa ccctcactaa
      361 agggaacaaa agctgggtac cgggcccccc ctcgaggtcg acggtatcga taagcttgat
      421 atcgaattcc tgcagcccgg gggatccact agttctagag cggccgccac cgcggtggag
      481 ctccaattcg ccctatagtg agtcgtatta ataacttcgt atagcataca ttatacgaag
      541 ttattatatg aagattgttc taccattcac aactatatga aaggccacag aagaaaatta
      601 ccttccatgt ggatgacgat atgcgtagca tggaaatgta tcatgaataa aatattgttc
      661 ctcagtcaag cacataccgg aaagacaact gcgatttaat aagagcaaga aggtgataaa
      721 acctactcac tcaaatgcct ctattgtaaa ggaaaggaca accctcattc caaaatttaa
      781 atttatcaat attctcatt
//
