LOCUS       sequence-166606-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Tagging vector HIS/iRFP - Plasmid for tandem infrared RFP 
            (tdiRFP) labeling of endogenous proteins with a HIS selection 
            marker, originating from pSIVh (ID:81091).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTH-ir
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..20
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     60..76
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     61..77
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     105..121
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     105..121
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 aaattaaccc tcactaaagg ggcatgcaac aaaagctggg tacggtaccg ggccccccct
       61 cgaggtcgac ggtatcgata agcttctcaa gaactggcgg ccgctctaga actagtggat
      121 cctctagaac taatggcaga gggaagcgtt gcccgtcaac ccgacctatt aacttgcgat
      181 gatgaaccta tccatatccc cggagcaatt caacctcacg gtctattgtt agcccttgct
      241 gccgatatga caatcgttgc aggttcagat aacctaccgg aattgaccgg gttagccatt
      301 ggagccttga tcggccgttc agccgcagat gtgttcgatt cagaaacaca taatagactg
      361 accatcgcct tggctgaacc aggggccgct gttggcgctc ccattacagt tggcttcacg
      421 atgagaaagg atgctggttt tattggatca tggcatcgtc atgatcaact tattttcttg
      481 gaattggaac ctcctcaaag ggatgttgct gaacctcagg ctttctttcg tcgtactaat
      541 agcgctattc gtagacttca ggctgctgaa acattggaga gcgcttgcgc agcagcagct
      601 caagaagtta gaaagattac cggttttgac agagtaatga tatatagatt tgcgtccgat
      661 ttctctggtg aagttattgc ggaagataga tgtgccgagg tagagtcaaa gttggggtta
      721 cattatcctg cttcaacggt tccagcacaa gcaaggcgtc tgtacacaat aaaccccgta
      781 agaataattc cagacatcaa ctacagacct gtcccagtaa ctcctgatct gaaccctgtg
      841 actggtagac ctatagattt atcttttgca attttgagat cagtgtcacc ggttcacttg
      901 gagttcatga gaaatattgg tatgcacgga accatgtcca tttctatact gaggggcgaa
      961 aggttgtggg ggctgattgt ttgccatcat ag
//
