LOCUS       sequence-16661-e        1350 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L2822
FEATURES             Location/Qualifiers
     source          1..1350
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    29..136
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     53..69
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     53..69
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(103..119)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(104..120)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(177..205)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gnttaccctc ctaagggaac aaaagctgga gctccaccgc ggtggcggcc gctctagaac
       61 tagtggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga
      121 gggggggccc ggtaccggta gaaaaaatga gtaaaggaga agaacttttc actggagttg
      181 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
      241 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
      301 aactacctgt tccatgggta agtttaaaca tatatatact aactaaccct gattatttaa
      361 attttcagcc aacacttgtc actactttct gttatggtgt tcaatgcttc tcgagatacc
      421 cagatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      481 aaagaactat atttttcaaa gatgacggga actacaagac acgtaagttt aaacagttcg
      541 gtactaacta accatacata tttaaatttt caggtgctga agtcaagttt gaaggtgata
      601 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
      661 gacacaaatt ggaataccaa ctataactca cacaatgtat acatcatggc agacaaacaa
      721 aagaatggaa tcaaagttgt aagtttaaac atgattttac taactaacta atctgattta
      781 aattttcaga acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      841 cattatcaac aaaatactcc aattggcgat ggccctgtcc cttttaccag acaaccatta
      901 cctgtccaca caatctgccc tttcgaanga tccccacgaa aagagagacc acatggtcct
      961 tcttgagttg taacagctgc tgggatacac atggcatgga tgaactatac aatgnccggg
     1021 ggatcggngg anctcccccg cggnggcggc cgctctanaa ctagnggatc ccccgggctg
     1081 caggantcna aatcagctta tcgatncncc caccncnagg gggggccggg ncccaattcc
     1141 cccnaaaggn ggcnaatacn cncccnccng gnccnctttt aaacgncggg acgggaaaac
     1201 ccgggggtac ccanttantc ccntggaaca nccccctttc cccggnggga aanacaaaan
     1261 ncccccccat ncccctccna aatngccccc gangggaang nacccccnng gggccntann
     1321 cccggggggg ggtncccccc ccccccctcc
//
