LOCUS       sequence-166610-        5754 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene Tagging vector HIS/iRFP - Plasmid for tandem infrared RFP 
            (tdiRFP) labeling of endogenous proteins with a HIS selection 
            marker, originating from pSIVh (ID:81091).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Wosika V, Durandau E, Varidel C, Aymoz D, Schmitt M, Pelet S
  TITLE     New families of single integration vectors and gene tagging plasmids
            for genetic manipulations in budding yeast.
  JOURNAL   Mol Genet Genomics. 2016 Dec;291(6):2231-2240. Epub 2016 Sep 16.
  PUBMED    27637489
REFERENCE   2  (bases 1 to 5754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2016-12"; volume: "291(6)"; pages: "2231-2240. 
            Epub 2016 Sep 16"
COMMENT     Sequence Label: GTH-ir
FEATURES             Location/Qualifiers
     source          1..5754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(22..85)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        112..130
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     170..186
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     171..187
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     215..231
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     215..231
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2366..2553
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2421..2445)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(2592..2608)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2592..2608)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2609..2642
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3144..3797
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MGRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQ
                     KGEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQ
                     AMGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLY
                     SFSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     terminator      3803..4000
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    4024..4057
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4058..4076
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4105..4123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4156..4744)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4236..4255)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4915..5706,5707..5754,1..21))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5538..5557
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
       61 ttattgtctc atgagcggat acatatttga agtatcgttc gaattaatta aaattaaccc
      121 tcactaaagg ggcatgcaac aaaagctggg tacggtaccg ggccccccct cgaggtcgac
      181 ggtatcgata agcttctcaa gaactggcgg ccgctctaga actagtggat cctctagaac
      241 taatggcaga gggaagcgtt gcccgtcaac ccgacctatt aacttgcgat gatgaaccta
      301 tccatatccc cggagcaatt caacctcacg gtctattgtt agcccttgct gccgatatga
      361 caatcgttgc aggttcagat aacctaccgg aattgaccgg gttagccatt ggagccttga
      421 tcggccgttc agccgcagat gtgttcgatt cagaaacaca taatagactg accatcgcct
      481 tggctgaacc aggggccgct gttggcgctc ccattacagt tggcttcacg atgagaaagg
      541 atgctggttt tattggatca tggcatcgtc atgatcaact tattttcttg gaattggaac
      601 ctcctcaaag ggatgttgct gaacctcagg ctttctttcg tcgtactaat agcgctattc
      661 gtagacttca ggctgctgaa acattggaga gcgcttgcgc agcagcagct caagaagtta
      721 gaaagattac cggttttgac agagtaatga tatatagatt tgcgtccgat ttctctggtg
      781 aagttattgc ggaagataga tgtgccgagg tagagtcaaa gttggggtta cattatcctg
      841 cttcaacggt tccagcacaa gcaaggcgtc tgtacacaat aaaccccgta agaataattc
      901 cagacatcaa ctacagacct gtcccagtaa ctcctgatct gaaccctgtg actggtagac
      961 ctatagattt atcttttgca attttgagat cagtgtcacc ggttcacttg gagttcatga
     1021 gaaatattgg tatgcacgga accatgtcca tttctatact gaggggcgaa aggttgtggg
     1081 ggctgattgt ttgccatcat aggacaccat attatgtgga cttagacggt aggcaggcgt
     1141 gcgagctggt cgcccaagtt ttggcttggc agattggtgt catggaagag aaggagggcc
     1201 acggcaccgg cagcaccggc agcggcagca gcggcagaag cggcaccgcc agcagcgagg
     1261 acaacaacat ggccagaatg ctagaactaa tggccgaagg tagcgttgca cgtcaacccg
     1321 atctactaac atgcgatgat gaacctatac atattcctgg ggctattcaa cctcatggtt
     1381 tgttattggc attggcagct gatatgacga tcgttgctgg aagcgacaat ttgccggagt
     1441 taactggact agccatcggt gctcttatag gtagatccgc tgctgacgtt ttcgattcag
     1501 aaacgcataa caggctaacg atcgctcttg ctgagcctgg tgcagccgtt ggagcaccca
     1561 tcaccgttgg ttttactatg agaaaggatg ctggttttat tggttcctgg catagacatg
     1621 accaattaat attcttagaa ttggaacctc cccagaggga tgtcgcggaa ccacaagcat
     1681 tttttagaag aacaaattca gcgattagaa gattacaagc agctgaaaca cttgaatcag
     1741 catgtgcagc agctgcacag gaggtaagga agattacggg atttgacaga gtaatgatct
     1801 atagatttgc tagtgacttc agtggcgaag ttatagctga agatagatgt gcggaggttg
     1861 agagtaaact gggtttacat tatccagctt ccacagttcc agcgcaagca aggagattat
     1921 acactataaa cccagtaagg atcatcccag acattaacta cagaccggtc ccagtaactc
     1981 ctgatttaaa cccagtaacc ggtagaccta ttgacttgtc tttcgcaatt ttaaggtctg
     2041 tgtccccagt ccatttggag tttatgagaa atatagggat gcatgggacg atgtcaattt
     2101 ctatactgcg tggtgagaga ctgtggggac ttattgtttg ccatcataga actccatatt
     2161 atgtggatct ggacggtaga caggcgtgtg aacttgtggc ccaggtgtta gcttggcaga
     2221 tcggagttat ggaagagaag gagggccacg gcaccggcag caccggcagc ggcagcagcg
     2281 gcagaagcgg caccgccagc agcgaggaca acaacatggc cagaatgcta gcattttaac
     2341 tcgagggcgc gccacttcta aataagcgaa tttcttatga tttatgattt ttattattaa
     2401 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg
     2461 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagta
     2521 tgaggtcgct cttattgacc acacctctac cggcagatcc gctagggata acagggtaat
     2581 atagatccat ggtcatagct gtttcctgat aacttcgtat aatgtatgct atacgaagtt
     2641 atgtctgtag gcgaacctaa ccggccaatt ttccaagacc gctctgccgc tgagcttgca
     2701 cccgtgccgc tacacaacac ccacacatcc gtaaaccccc cgccatcggt atcaccaagg
     2761 ttgtctatga cgaggcgtac aagaggacgt gggggccgcc tctccaatgt caccaatagg
     2821 aaaaaagaaa gaccgagtgc gagtttctga cagaagcctc aagaattttt tttctttctt
     2881 cgactatgct ggaggtagag atgatcgagg cagtagtcaa gtatatataa attaattagt
     2941 tccaccacct tcttttcttg tgttgctcct ttcgatgcta tttctggctt tttctaattt
     3001 ttttcatcgt ttcttacttt cttcctaata tataaattct tttgcatttt cattcttcct
     3061 ctccatttgt tcattctcct taaatttttt ttaggtctcc tttctttcaa gaaacacagt
     3121 catcgaacta taattaacta aacatgggta ggagggcttt tgtagaaaga aatacgaacg
     3181 aaacgaaaat cagcgttgct atcgctttgg acaaagctcc cttacctgaa gagtcgaatt
     3241 ttattgatga acttataact tccaaacatg caaaccaaaa gggagaacaa gtaatccaag
     3301 tagacacggg aattggattc ttggatcaca tgtatcatgc actggctaaa catgcaggct
     3361 ggagcttacg actttactca agaggtgatt taatcattga tgatcatcac acagcagaag
     3421 atactgctat tgcacttggt attgcattca agcaggctat gggtaacttt gccggcgtta
     3481 aaagatttgg acatgcttat tgtccacttg acgaagctct ttcaagaagc gtagttgatt
     3541 tgtcgggacg gccctatgct gttattgatt tgggattaaa gcgtgaaaag gttggggaat
     3601 tgtcctgtga aatgatccct cacttactat attccttttc ggtagctgct ggaattactt
     3661 tgcatgttac ctgcttatat ggtagtaatg accatcatcg tgctgaaagc gcttttaaat
     3721 ctctggctgt tgccatgcgc gcggctacaa gtcttactgg aagttctgaa gtcccaagca
     3781 cgaagggagt gttgtaatca ttactgacaa taaaaagatt cttgttttca agaacttgtc
     3841 atttgtatag tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta
     3901 tatttttttt cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat
     3961 atcatgcgtc aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg
     4021 ccgataactt cgtataatgt atgctatacg aagttattaa tacgactcac tatagggcga
     4081 attggagctc caattcggac gtcccctata gtgagtcgta ttattaatta attcgaaggc
     4141 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4201 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4261 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4321 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4381 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4441 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4501 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4561 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4621 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4681 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4741 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4801 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4861 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4921 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4981 ctgactgccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5041 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5101 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5161 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5221 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     5281 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgaaaaa aagcggttag
     5341 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5401 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5461 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     5521 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5581 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5641 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5701 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgac
//
