LOCUS       sequence-166613-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shuttle plasmid for sarA P1-dsRED expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   de Jong NW, van der Horst T, van Strijp JA, Nijland R
  TITLE     Fluorescent reporters for markerless genomic integration in 
            Staphylococcus aureus.
  JOURNAL   Sci Rep. 2017 Mar 7;7:43889. doi: 10.1038/srep43889.
  PUBMED    28266573
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-7"; volume: "7:43889. doi"; pages: " 
            10.1038/srep43889"
COMMENT     Sequence Label: pTH1
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..686)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(71..94)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(269..288)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     486..506
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     538..556
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 acgaattcct acaggaacag gtggtggcgg ccctcggtgc gctcgtactg ctccacgatg
       61 gtgtagtcct cgttgtggga ggtgatgtcc agcttggagt ccacgtagta gtagccgggc
      121 agctgcacgg gcttcttggc catgtagatg gacttgaact ccaccaggta gtggccgccg
      181 tccttcagct tcagggcctt gtggatctcg cccttcagca cgccgtcgcg ggggtacagg
      241 cgctcggtgg agggctccca gcccatagtc ttcttctgca ttacggggcc gtcggagggg
      301 aagttcacgc cgatgaactt caccttgtag atgaagcagc cgtcctgcag ggaggagtcc
      361 tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc
      421 tcggggaagg acagcttctt gtagtcgggg atgtcggcgg ggtgcttcac gtacaccttg
      481 gagccgtact ggaactgggg ggacaggatg tcccaggcga agggcagggg gccgcccttg
      541 gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg
      601 atctcgaact cgtggccgtt cacggagccc tccatgcgca ccttgaagcg catgaactcc
      661 ttgatgacgt cctcggtgtt gtccatatgt ttttcctcct ggtacccggg gatccgatgc
      721 atcttgctcg atacatttgc ccgataatat attgatatct aatctttatt tattatagat
      781 atgttagtca taattttgca ttaaataagt tttattaaat atatttaatg ctctattatt
      841 t
//
