LOCUS       sequence-166614-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shuttle plasmid for sarA P1-dsRED expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   de Jong NW, van der Horst T, van Strijp JA, Nijland R
  TITLE     Fluorescent reporters for markerless genomic integration in 
            Staphylococcus aureus.
  JOURNAL   Sci Rep. 2017 Mar 7;7:43889. doi: 10.1038/srep43889.
  PUBMED    28266573
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-7"; volume: "7:43889. doi"; pages: " 
            10.1038/srep43889"
COMMENT     Sequence Label: pTH1
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(681..695)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
       61 cgaactcgtg gccgttcacg gagccctcca tgcgcacctt gaagcgcatg aactccttga
      121 tgacgtcctc ggtgttgtcc atatgttttt cctcctggta cccggggatc cgatgcatct
      181 tgctcgatac atttgcccga taatatattg atatctaatc tttatttatt atagatatgt
      241 tagtcataat tttgcattaa ataagtttta ttaaatatat ttaatgctct attatttagt
      301 taattataac taattaaaaa tgagaagtaa acaaaaaagt gtttataaaa caaattattc
      361 attagacaca gtgattgtat ttctgggtta gcatttggtt tagtcaaaaa tatcaggcta
      421 gcggatcctc tagagtcgac ctgcaggcat gcaagctttt aaaaagcaaa tatgagccaa
      481 ataaatatat tctaattcta caaacaaaaa tttgagcaaa ttcagtgtcg attttttaag
      541 acactgccca gttacatgca aattaaaatt ttcatgattt tttatagttc ctaacagggt
      601 taaaatttgt ataacgaaag tataatgttt atataacgtt agtataataa agcattttaa
      661 cattatactt ttgataatcg tttatcgtcg tcatcacaat aacttttaaa atactcgtgc
      721 ataattcaac agctgacctc ccaataacta catggtgtta tcgggaggtc agctgttagc
      781 acttatattt tgttattgtt cttcctcgat ttcgtctatc attttgtgat taatttctct
      841 tttttcttgt tctgttaagt cataaagttc actagctaaa tactc
//
