LOCUS       sequence-166616-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shuttle plasmid for sarA P1-CFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   de Jong NW, van der Horst T, van Strijp JA, Nijland R
  TITLE     Fluorescent reporters for markerless genomic integration in 
            Staphylococcus aureus.
  JOURNAL   Sci Rep. 2017 Mar 7;7:43889. doi: 10.1038/srep43889.
  PUBMED    28266573
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-7"; volume: "7:43889. doi"; pages: " 
            10.1038/srep43889"
COMMENT     Sequence Label: pTH2
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(51..71)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     666..694
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgaattctta cttgtacagc tcgtccatgc caagtgtaat cccagcagct gttacaaact
       61 caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagttta gattgtgtgg
      121 acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat
      181 aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg aagttagctt
      241 tgattccatt cttttgtttg tctgctgtga tgtatacatt atctgatata gcgttgtatt
      301 ccaatttgtg tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc
      361 tattaacaag ggtatcacct tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat
      421 ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaaaagt
      481 catgctgttt catatgatct gggtatctcg caaagcattg tacaccccat gtaagagtag
      541 tgacaagtgt tggccatgga acaggtagtt ttccagtagt gcaaataaat ttaagggtaa
      601 gttttccgta tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa
      661 catcaccatc taattcaaca agaattggga caactccagt gaaaagttct tctcctttac
      721 tcataaataa tcatcctcct aaggtacccg gggatccgat gcatcttgct cgatacattt
      781 gcccgataat atattgatat ctaatcttta tttattatag atatgttagt cataattttg
      841 cattaaataa gttttattaa atatatttaa tgctctatta tttagttaat tataactaat
      901 taaaaatgag aagtaaacaa aaaagtgttt ataaaacaaa ttattcatta ga
//
