LOCUS       sequence-166618-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shuttle plasmid for sarA P1-CFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   de Jong NW, van der Horst T, van Strijp JA, Nijland R
  TITLE     Fluorescent reporters for markerless genomic integration in 
            Staphylococcus aureus.
  JOURNAL   Sci Rep. 2017 Mar 7;7:43889. doi: 10.1038/srep43889.
  PUBMED    28266573
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-7"; volume: "7:43889. doi"; pages: " 
            10.1038/srep43889"
COMMENT     Sequence Label: pTH2
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(554..568)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 cctttactca taaataatca tcctcctaag gtacccgggg atccgatgca tcttgctcga
       61 tacatttgcc cgataatata ttgatatcta atctttattt attatagata tgttagtcat
      121 aattttgcat taaataagtt ttattaaata tatttaatgc tctattattt agttaattat
      181 aactaattaa aaatgagaag taaacaaaaa agtgtttata aaacaaatta ttcattagac
      241 acagtgattg tatttctggg ttagcatttg gtttagtcaa aaatatcagg ctagcggatc
      301 ctctagagtc gacctgcagg catgcaagct tttaaaaagc aaatatgagc caaataaata
      361 tattctaatt ctacaaacaa aaatttgagc aaattcagtg tcgatttttt aagacactgc
      421 ccagttacat gcaaattaaa attttcatga ttttttatag ttcctaacag ggttaaaatt
      481 tgtataacga aagtataatg tttatataac gttagtataa taaagcattt taacattata
      541 cttttgataa tcgtttatcg tcgtcatcac aataactttt aaaatactcg tgcataattc
      601 aacagctgac ctcccaataa ctacatggtg ttatcgggag gtcagctgtt agcacttata
      661 ttttgttatt gttcttcctc gatttcgtct atcattttgt gattaatttc tcttttttct
      721 tgttctgtta agtcataaag ttcactagct aaatactctt tttgtttcca aatataaaaa
      781 atttgataga tatattcggt tggatcaatt tcttttaagt aatctaaatc cccatttttt
      841 aatttc
//
