LOCUS       sequence-166623-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shuttle plasmid for sarA P1-mCherry expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   de Jong NW, van der Horst T, van Strijp JA, Nijland R
  TITLE     Fluorescent reporters for markerless genomic integration in 
            Staphylococcus aureus.
  JOURNAL   Sci Rep. 2017 Mar 7;7:43889. doi: 10.1038/srep43889.
  PUBMED    28266573
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-7"; volume: "7:43889. doi"; pages: " 
            10.1038/srep43889"
COMMENT     Sequence Label: pRN11
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(512..526)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 ccgatgcatc ttgctcgata catttgcccg ataatatatt gatatctaat ctttatttat
       61 tatagatatg ttagtcataa ttttgcatta aataagtttt attaaatata tttaatgctc
      121 tattatttag ttaattataa ctaattaaaa atgagaagta aacaaaaaag tgtttataaa
      181 acaaattatt cattagacac agtgattgta tttctgggtt agcatttggt ttagtcaaaa
      241 atatcaggct agcggatcct ctagagtcga cctgcaggca tgcaagcttt taaaaagcaa
      301 atatgagcca aataaatata ttctaattct acaaacaaaa atttgagcaa attcagtgtc
      361 gattttttaa gacactgccc agttacatgc aaattaaaat tttcatgatt ttttatagtt
      421 cctaacaggg ttaaaatttg tataacgaaa gtataatgtt tatataacgt tagtataata
      481 aagcatttta acattatact tttgataatc gtttatcgtc gtcatcacaa taacttttaa
      541 aatactcgtg cataattcaa cagctgacct cccaataact acatggtgtt atcgggaggt
      601 cagctgttag cacttatatt ttgttattgt tcttcctcga tttcgtctat cattttgtga
      661 ttaatttctc ttttttcttg ttctgttaag tcataaagtt cactagctaa atactctttt
      721 tgtttccaaa tataaaaaat ttgatagata tattcggttg gatcaatttc ttttaagtaa
      781 tctaaatccc cattttttaa tttcttttta gcctctttaa ataatcctga ataaactaat
      841 acctgtttac ctttaagtga tttataaaat gcatcaaaga ctttttgatt tattaaataa
      901 tcactatctt taccagaata cttagccatt tcatataatt cttt
//
