LOCUS       sequence-16663-e        1295 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L2908
FEATURES             Location/Qualifiers
     source          1..1295
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(139..155)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(140..156)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(253..281)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gnttacctcc taaagggaac aaaagctgga gctccaccgc ggtggcggcc gctctagaac
       61 tagnggatcc accggttcca tggctagcca cgtgacgcgt ggatcccccg ggctgcagga
      121 attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta cgatatacta
      181 actaacaaga attatctaat ttttcaggta ccggtagaaa aaatgagtaa aggagaagaa
      241 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
      301 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
      361 atttgcacta ctggaaaact acctgttcca tgggtaagtt taaacatata tatactaact
      421 aaccctgatt atttaaattt tcagccaaca cttgtcacta ctttctgtta tggtgttcaa
      481 tgcttctcga gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc
      541 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
      601 aagtttaaac agttcggtac taactaacca tacatattta aattttcagg tgctgaagtc
      661 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
      721 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
      781 atggcagaca aacaaaagaa tggaatcaaa gttgtaagtt taaacatgat tttactaact
      841 aactaatctg atttaaattt tcagaacttc aaaattagac acaacattga aagatggaag
      901 cgttcaacta gcagaccatt atcacaaaat actccaattg gcgatggccc tgtcctttta
      961 ccagacaccn ttacctgtcc cncaatctgc cctttcgaag atccnacgaa aaganaaacc
     1021 catggtcctc ctgagttgna acagctgctg ggataccctg gcnggangaa ctnnacaagt
     1081 agtttacntt tatcntacna atgaaancnc cccgcggggg gcncnnaaan tagggatccc
     1141 ggtccngnta ccccggaccg gnaccccggn gnngatncaa tcacttacaa cnccacccng
     1201 ggggccggnc natccccaaa gngcnanacc ncccggcncn ttaacgcggn gggaaccggg
     1261 tncnatancc tgncacccct cccngggaan aaanc
//
