LOCUS       sequence-166630-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pJB38-NWMN29-30 + SarA_P1-sGFP-Term.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   de Jong NW, van der Horst T, van Strijp JA, Nijland R
  TITLE     Fluorescent reporters for markerless genomic integration in 
            Staphylococcus aureus.
  JOURNAL   Sci Rep. 2017 Mar 7;7:43889. doi: 10.1038/srep43889.
  PUBMED    28266573
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-7"; volume: "7:43889. doi"; pages: " 
            10.1038/srep43889"
COMMENT     Sequence Label: pTH100
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(40..750,751..753))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(40..753)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(80..100)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     695..723
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gattacgaat tcttagtggt ggtggtggtg gtgggatcct ttgtagagct catccatgcc
       61 atgtgtaatc ccagcagcag ttacaaactc aagaaggacc atgtggtcac gcttttcgtt
      121 aggatctttc gaaaggacag attgtgtcga caggtaatgg ttgtctggta aaaggacagg
      181 gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgg aaccatcttc
      241 aacgttgtgg cgaattttga agttagcttt gattccattc ttttgtttgt ctgccgtgat
      301 gtatacattg tgtgagttaa agttgtactc gagtttgtgt ccaagaatgt ttccatcttc
      361 tttaaaatca atacccttta actcgatacg attaacaagg gtatcacctt caaacttgac
      421 ttcagcacgc gtcttgtagg tcccgtcatc tttgaaagat atagtgcgtt cctgtacata
      481 accttcaggc atggcactct tgaaaaagtc atgacgtttc atgtgatccg gataacggga
      541 aaagcattga acaccatagg tcagagtagt gacaagtgtt ggccacggaa caggtagttt
      601 tccagtagtg caaataaatt taagggtgag ttttccgttt gtagcatcac cttcaccctc
      661 tccacggaca gaaaatttgt gcccattaac atcaccatct aattcaacaa gaattgggac
      721 aactccagtg aaaagttctt ctcctttgct cataaataat catcctccta aggtacccgg
      781 ggatccgatg catcttgctc gatacatttg cccgataata tattgatatc taatctttat
      841 ttattataga tatgttagtc ataattttgc attaa
//
