LOCUS       sequence-166632-         352 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation plasmid for phage display on modified geneIII, 
            contains stuffer fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Andris-Widhopf J, Rader C, Steinberger P, Fuller R, Barbas CF 3rd
  TITLE     Methods for the generation of chicken monoclonal antibody fragments 
            by phage display.
  JOURNAL   J Immunol Methods. 2000 Aug 28;242(1-2):159-81.
  PUBMED    10986398
REFERENCE   2  (bases 1 to 352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Immunol 
            Methods."; date: "2000-08-28"; volume: "242(1-2)"; pages: "159-81"
COMMENT     Sequence Label: pComb3XSS
FEATURES             Location/Qualifiers
     source          1..352
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(122..127,128..145,146..152)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    160..176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     sig_peptide     199..261
                     /codon_start=1
                     /product="signal peptide from the E. coli outer membrane 
                     protein OmpA"
                     /label=OmpA signal peptide
                     /translation="MKKTAIAIAVALAGFATVAQA"
     sig_peptide     complement(271..336)
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
ORIGIN
        1 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
       61 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
      121 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaattga
      181 attcaggagg aatttaaaat gaaaaagaca gctatcgcga ttgcagtggc actggctggt
      241 ttcgctaccg tggcccaggc ggccgagctc ggccatggct ggttgggcag cgagtaataa
      301 caatccagcg gctgccgtag gcaataggta tttcattatg actgtctcct tg
//
