LOCUS       sequence-166633-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pJB38-NWMN29-30 + SarA_P1-DsRed-Term.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   de Jong NW, van der Horst T, van Strijp JA, Nijland R
  TITLE     Fluorescent reporters for markerless genomic integration in 
            Staphylococcus aureus.
  JOURNAL   Sci Rep. 2017 Mar 7;7:43889. doi: 10.1038/srep43889.
  PUBMED    28266573
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-7"; volume: "7:43889. doi"; pages: " 
            10.1038/srep43889"
COMMENT     Sequence Label: pTH101
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..691)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(76..99)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(274..293)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     491..511
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     543..561
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tgattacgaa ttcctacagg aacaggtggt ggcggccctc ggtgcgctcg tactgctcca
       61 cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggagtccacg tagtagtagc
      121 cgggcagctg cacgggcttc ttggccatgt agatggactt gaactccacc aggtagtggc
      181 cgccgtcctt cagcttcagg gccttgtgga tctcgccctt cagcacgccg tcgcgggggt
      241 acaggcgctc ggtggagggc tcccagccca tagtcttctt ctgcattacg gggccgtcgg
      301 aggggaagtt cacgccgatg aacttcacct tgtagatgaa gcagccgtcc tgcagggagg
      361 agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc tcccacttga
      421 agccctcggg gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc ttcacgtaca
      481 ccttggagcc gtactggaac tggggggaca ggatgtccca ggcgaagggc agggggccgc
      541 ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc
      601 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gcgcaccttg aagcgcatga
      661 actccttgat gacgtcctcg gtgttgtcca tatgtttttc ctcctggtac ccggggatcc
      721 gatgcatctt gctcgataca tttgcccgat aatatattga tatctaatct ttatttatta
      781 tagatatgtt agtcataatt ttgcattaaa taagttttat taaatatatt taatgctcta
      841 ttatttagtt aattataact aatt
//
