LOCUS       sequence-166636-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pJB38-NWMN29-30 + SarA_P1-CFP-Term.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   de Jong NW, van der Horst T, van Strijp JA, Nijland R
  TITLE     Fluorescent reporters for markerless genomic integration in 
            Staphylococcus aureus.
  JOURNAL   Sci Rep. 2017 Mar 7;7:43889. doi: 10.1038/srep43889.
  PUBMED    28266573
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-7"; volume: "7:43889. doi"; pages: " 
            10.1038/srep43889"
COMMENT     Sequence Label: pTH102
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(57..77)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     672..700
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tgattacgaa ttcttacttg tacagctcgt ccatgccaag tgtaatccca gcagctgtta
       61 caaactcaag aaggaccatg tggtctctct tttcgttggg atctttcgaa agtttagatt
      121 gtgtggacag gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt
      181 gttgataatg gtctgctagt tgaacgcttc catcttcaat gttgtgtcta attttgaagt
      241 tagctttgat tccattcttt tgtttgtctg ctgtgatgta tacattatct gatatagcgt
      301 tgtattccaa tttgtgtcca agaatgtttc catcttcttt aaaatcaata ccttttaact
      361 cgattctatt aacaagggta tcaccttcaa acttgacttc agcacgtgtc ttgtagttcc
      421 cgtcatcttt gaaaaatata gttctttcct gtacataacc ttcgggcatg gcactcttga
      481 aaaagtcatg ctgtttcata tgatctgggt atctcgcaaa gcattgtaca ccccatgtaa
      541 gagtagtgac aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa
      601 gggtaagttt tccgtatgtt gcatcacctt caccctctcc actgacagaa aatttgtgcc
      661 cattaacatc accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc
      721 ctttactcat aaataatcat cctcctaagg tacccgggga tccgatgcat cttgctcgat
      781 acatttgccc gataatatat tgatatctaa tctttattta ttatagatat gttagtcata
      841 attt
//
