LOCUS       sequence-16666-e        1370 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L2946
FEATURES             Location/Qualifiers
     source          1..1370
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             359..379
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(422..450)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtgctgcggt cgactctaga ggatccccaa ttcttgagac ttccactatc ttctctggca
       61 ccctccacta cgcgtaggcg tctctcgctt cgtgtattcc cgggaagccg ggttcccgtc
      121 tctcccgccg ctgccgctgc cgcacacagc tttacacctc gtagaatccc caaagagggg
      181 cgtggcttgc gggtgccaac atcctcctgc cgaggaagaa gcaggcactc atcactcgca
      241 tcatcaacct cgggattggc caaaggaccc aaaggtatgt ttcgaatgat actaacataa
      301 catagaacat tttcaggagg acccttggag ggtaccgagc tcagaaaaaa tgactgctcc
      361 aaagaagaag cgtaaggtac cggtagaaaa aatgagtaaa ggagaagaac ttttcactgg
      421 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      481 tggagagggt gaaggtgatg taacatacgg aaaacttacc cttaaattta tttgcactac
      541 tggaaaacta cctgttccat gggtaagttt aaacatatat atactaacta accctgatta
      601 tttaaatttt cagccaacac ttgtcactac tttcgcttat ggtcttcaat gcttcgccag
      661 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
      721 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgta agtttaaaca
      781 gttcggtact aactaaccat acatatttaa attttcaggt gctgaagtca agtttgaagg
      841 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
      901 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
      961 acaaaagaat ggaatcaaag ttgtaagttn aacatgantt tactanctaa ctaatctgat
     1021 ttaaattttc anaacttcaa attagacccc accatngaan atggaagcgt tcactagcan
     1081 accnttntca acaaaatacn ccantgggng anggcctgtc ttttaccana aancattact
     1141 gtccnccaat ntgncctttc aaaantccaa gaaaaanaac ncntggncct ntgaatttga
     1201 aaccgggggn atacnngggc nggaaaacna caaanncctc ngnaatgnag tttaacntnc
     1261 caacaaaanc cccttttant ttaaantcca ggagcnngcc nnatannttn gngggnaaaa
     1321 aggnccnccc ccaaaattan gttccnaana naattnnttc ccccncnccc
//
