LOCUS       sequence-166673-        1024 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To study the role of the differential rates of G-beta-gamma 
            translocation in modulating signaling dynamics in a cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Giri L, Patel AK, Karunarathne WK, Kalyanaraman V, Venkatesh KV, 
            Gautam N
  TITLE     A G-protein subunit translocation embedded network motif underlies 
            GPCR regulation of calcium oscillations.
  JOURNAL   Biophys J. 2014 Jul 1;107(1):242-54. doi: 10.1016/j.bpj.2014.05.020.
  PUBMED    24988358
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2014.05"; journalName: "Biophys J"; date: "2014-07-1-
            1"; volume: "107"; issue: "1"; pages: "242-54"
COMMENT     Sequence Label: pcDNA3.1 mCherry Gamma 3
FEATURES             Location/Qualifiers
     source          1..1024
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..730
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(168..186)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     436..455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gcttggtacc gagctcggat ccatggtgag caagggcgag gaggataaca tggccatcat
       61 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      181 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
      241 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
      421 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      481 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
      541 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
      601 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
      721 gctgtacaag ggtaccatga aaggggagac ccccgttaac agcactatga gtattggtca
      781 agcacgcaag atggtggaac agcttaagat tgaggccagc ttgtgccgga taaaggtgtc
      841 caaggcagca gcagacctga tgacatactg tgatgcccac gcttgtgagg accccctcat
      901 cactcctgtg cccacttccg agaacccctt ccgggagaag aagttcttct gtgcgctcct
      961 ctgagaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta
     1021 aacc
//
