LOCUS       sequence-16668-e        1300 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L2963
FEATURES             Location/Qualifiers
     source          1..1300
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             359..379
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(422..450)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gngctgcggt cgactctaga ggatccccaa ttcttgagac ttccactatc ttctctggca
       61 ccctccacta cgcgtaggcg tctctcgctt cgtgtattcc cgggaagccg ggttcccgtc
      121 tctcccgccg ctgccgctgc cgcacacagc tttacacctc gtagaatccc caaagagggg
      181 cgtggcttgc gggtgccaac atcctcctgc cgaggaagaa gcaggcactc atcactcgca
      241 tcatcaacct cgggattggc caaaggaccc aaaggtatgt ttcgaatgat actaacataa
      301 catagaacat tttcaggagg acccttggag ggtaccgagc tcagaaaaaa tgactgctcc
      361 aaagaagaag cgtaaggtac cggtagaaaa aatgagtaaa ggagaagaac ttttcactgg
      421 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      481 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      541 tggaaaacta cctgttccat gggtaagttt aaacatatat atactaacta accctgatta
      601 tttaaatttt cagccaacac ttgtcactac tttctgttat ggtgttcaat gcttctcgag
      661 atacccagat catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt
      721 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgta agtttaaaca
      781 gttcggtact aactaaccat acatatttaa attttcaggt gctgaagtca agtttgaagg
      841 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
      901 tcttggacac aaattggaat acaactataa ctcacacatg tatacatcat gggcagacaa
      961 acaaaagaat ggaatcaaag ttgtaagttt aaacatgatt ttactaacta actaatctga
     1021 nttaaatttt ccnaacttcn aaattanacc naccnttgaa gatggaagcg ttcaactagc
     1081 aaaccattat caacaaaata ctccaatngg ngangggccn ngtcntttta cnnanaaccn
     1141 ttatctaagg naantttaaa ggcnngggga cnaacgttgc ctagcnctac naaaanaact
     1201 ggaantaana anttttnanc acnnaatctg nccntttcca aaatnccaac gaaaanaaaa
     1261 accncnggnc cctctngant tnnaancccc ggngggaana
//
