LOCUS       sequence-166685-       11566 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA843  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11566)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM843
FEATURES             Location/Qualifiers
     source          1..11566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        156..501
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    662..838
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1400..1418)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1401..1418)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2057..2076
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2179..2184,2185..2201,2202..2207))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2482..2862
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2868..3419
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3429..3681
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    3767..3791
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4216..5010
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5097..5685
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5839..5856
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5871..6011
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5937..5956)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6097..6119
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6355..6549
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6615..7688)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8117..8746)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10047..10071
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10259..10281
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10273..10290
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10274..10290
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10315..11022)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     11296..11315
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11296..11314
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cccacgagga gcatcgtggg aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
       61 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat
      121 gtcaaagata ctgtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt
      181 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acctcatcga aaggacagta
      241 gaaaaggaag gtggctccta caaatgccat cattgcaata aaggaaaggc tatcattcaa
      301 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      361 aaaggagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac
      421 ataagggatg acgcacaacc ccactatcct ttgcaagacc cttcctctat ataaggaagt
      481 tcatttcatt tggagaggac agcccaagct ttccaatgaa gcctatcgac ctaaagttgt
      541 tgttgttatg gtctaattta aatatggtct aaagaagaag aattccaatg aagcctatcg
      601 acctaaagaa ttcggtacgc tgaaatcacc agtctctctc tacaaatcta tctctctcta
      661 ttttctccat aaataatgtg tgagtagctt cccgataagg gaaattaggg ttcttatagg
      721 gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata
      781 cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatctc
      841 ctaaagtccc tatagatctt tgtcgcgaat ataaaccaga cacgagacga ctagacctgg
      901 agcccagacg ccgttcgaag ctagaagtac cgcttaggca ggaggccgtt agggaaaaga
      961 tgctaaggca gggttggtta cgttgactcc cccgtaggtt tggtttaaat atgatgaagt
     1021 ggacggaagg aaggaggaag acaaggaagg ataaggttgc aggccctgtg caaggtaaga
     1081 agatggaaat ttgatagagg tacgctacta tacttatact atacgctaag ggaatgattg
     1141 tatttatacc ctataccccc taataacccc ttatcaattt aagaaataat ccgcataagc
     1201 ccccgcttaa aaattggtat cagagccatg aataggtcta tgaccaaaac tcaagaggat
     1261 aaaacctcac caaaatacga aagagtcctt aactctaaag ataaaagatc tttcaagatc
     1321 aaaactagtt ccctcacacc ggtgacaggg atcgcatgcg acctctagac catcacattg
     1381 gagcttcagc tgctcgagtt ctatagtgtc acctaaatcg tatgtgtatg atacataagg
     1441 ttatgtatta attgtagccg cgttcttacg tcgacgtacc aattacgccc cgccctgtca
     1501 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     1561 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     1621 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     1681 ggcgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
     1741 gaaataggcc aggttttcac cgtaacacgc cacgtcttgc gaatatatgt gtagaaactg
     1801 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     1861 aacggtgtaa caagggtgaa cactatccca tattaccagc tcaccgtctt tcattgccat
     1921 acggaactcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     1981 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     2041 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgctctttac gatgccattg
     2101 ggatatatca acggtggtat atccagtgat ttctttctcc attttagctt ccttagctcc
     2161 tgaaaatctc gataactcat taaagcttat cgatgataag ctgtcaaaca tgagaattta
     2221 gcccgcctaa tgagcgggct ttttgttgtg atccgcgttg ctggcgtttt tccataggct
     2281 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2341 aggactataa agataccagg cgtttccccc ctggaagctc cctcgtgcgc tctcctgttc
     2401 cgaccctgcc gcttaccgga tacctgtccg cctttcttaa ttaactctct agactcacct
     2461 aggatccatg aattctgaga tttttcaaat cagtgcgcaa gacgtgacgt aagtatccga
     2521 gtcagttttt atttttctac taatttggtc gtttatttcg gcgtgtagga catggcaacc
     2581 gggcctgaat ttcgcgggta ttctgtttct attccaactt tttcttgatc cgcagccatt
     2641 aacgactttt gaatagatac gctgacacgc caagcctcgc tagtcaaaag tgtaccaaac
     2701 aacgctttac agcaagaacg gaatgcgcgt gacgctcgcg gtgacgccat ttcgcctttt
     2761 cagaaatgga taaatagcct tgcttcctat tatatcttcc caaattacca atacattaca
     2821 ctagcatctg aatttcataa ccaatctcga tacaccaaat cgaatcgatg agcccagaac
     2881 gacgcccggc cgacatccgc cgtgccaccg aggcggacat gccggcggtc tgcaccatcg
     2941 tcaaccacta catcgagaca agcacggtca acttccgtac cgagccgcag gaaccgcagg
     3001 agtggacgga cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg
     3061 acggcgaggt cgccggcatc gcctacgcgg gcccctggaa ggcacgcaac gcctacgact
     3121 ggacggccga gtcgaccgtg tacgtctccc cccgccacca gcggacggga ctgggctcca
     3181 cgctctacac ccacctgctg aagtccctgg aggcacaggg cttcaagagc gtggtcgctg
     3241 tcatcgggct gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc
     3301 gcggcatgct gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg ggtttctggc
     3361 agctggactt cagcctgccg gtaccgcccc gtccggtcct gcccgtcacc gagatttgac
     3421 tgcagaatct tggactccca tgttggcaaa ggcaaccaaa caaacaatga atgatccgct
     3481 cctgcatatg gggcggtttg agtatttcaa ctgccatttg ggctgaattg aagacatgct
     3541 cctgtcagaa attccgtgat cttactcaat attcagtaat ctcggccaat atcctaaatg
     3601 tgcgtggctt tatctgtctt tgtattgttt catcaattca tgtaacgttt gcttttctta
     3661 tgaattttca aataaattat cagatccgag ctcgaattaa ttcggcgtta attcagtaca
     3721 ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat
     3781 atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac
     3841 tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc
     3901 agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga
     3961 aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc
     4021 ctgtgatcac cgcggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga
     4081 aaacaacttt gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga
     4141 attggagttc gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga
     4201 ggaaggaaat aataaatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa
     4261 aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg
     4321 ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat
     4381 gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag
     4441 gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc
     4501 gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat
     4561 gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc
     4621 ttagacagcc gcttagccga attggattac ttactgaata acgatctggc cgatgtggat
     4681 tgcgaaaact gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta
     4741 aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac
     4801 atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg
     4861 gacaagtggt atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa
     4921 cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa
     4981 tattatattt tactggatga attgttttag tacctagaat gcatgaccaa aatcccttaa
     5041 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5101 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5161 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5221 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     5281 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5341 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5401 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5461 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5521 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5581 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5641 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5701 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5761 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5821 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     5881 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     5941 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     6001 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     6061 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     6121 tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg
     6181 agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt
     6241 tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc
     6301 gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca
     6361 caggccaggc gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg
     6421 ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt
     6481 gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg
     6541 ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc
     6601 atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc
     6661 atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca
     6721 tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca
     6781 ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt
     6841 gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc
     6901 gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg
     6961 atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag
     7021 gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg
     7081 gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg
     7141 ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg
     7201 ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc
     7261 atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct
     7321 acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac
     7381 agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc
     7441 atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca
     7501 ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat
     7561 tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac
     7621 ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga
     7681 ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcaggcaa
     7741 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
     7801 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
     7861 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
     7921 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
     7981 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
     8041 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
     8101 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
     8161 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
     8221 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
     8281 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
     8341 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
     8401 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
     8461 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
     8521 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
     8581 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
     8641 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
     8701 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
     8761 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
     8821 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
     8881 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
     8941 tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
     9001 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
     9061 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
     9121 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
     9181 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
     9241 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
     9301 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
     9361 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
     9421 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
     9481 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
     9541 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
     9601 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
     9661 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
     9721 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
     9781 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
     9841 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
     9901 ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg
     9961 catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta
    10021 ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat
    10081 agtttaaact gaaggcggga aacgacaatc tgatccaagc tcaagctgct ctagcattcg
    10141 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
    10201 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
    10261 cagtcacgac gttgtaaaac gacggccagt gccaagcttg catgcctgca ggtcctgctg
    10321 agcctcgaca tgttgtcgca aaattcgccc tggacccgcc caacgatttg tcgtcactgt
    10381 caaggtttga cctgcacttc atttggggcc cacatacacc aaaaaaatgc tgcataattc
    10441 tcggggcagc aagtcggtta cccggccgcc gtgctggacc gggttgaatg gtgcccgtaa
    10501 ctttcggtag agcggacggc caatactcaa cttcaaggaa tctcacccat gcgcgccggc
    10561 ggggaaccgg agttcccttc agtgagcgtt attagttcgc cgctcggtgt gtcgtagata
    10621 ctagcccctg gggcactttt gaaatttgaa taagatttat gtaatcagtc ttttaggttt
    10681 gaccggttct gccgcttttt ttaaaattgg atttgtaata ataaaacgca attgtttgtt
    10741 attgtggcgc tctatcatag atgtcgctat aaacctattc agcacaatat attgttttca
    10801 ttttaatatt gtacatataa gtagtagggt acaatcagta aattgaacgg agaatattat
    10861 tcataaaaat acgatagtaa cgggtgatat attcattaga atgaaccgaa accggcggta
    10921 aggatctgag ctacacatgc tcaggttttt tacaacgtgc acaacagaat tgaaagcaaa
    10981 tatcatgcga tcataggcgt ctcgcatatc tcattaaagc aggactctag ggactagtcc
    11041 cgggtcttaa ttaagtttgc aagcagcaga ttaccggata cctgtccgcc tttcttaatt
    11101 aagtttgcaa gcagcagatt accggatacc tgtccgcctt tcttaattaa gtttgcaagc
    11161 agcagattac gcgcagaaaa aaaggatctc aaggagatcc tttggatcac aaaaaaaagc
    11221 ccgctcatta ggcgggttaa attcccgggt actgcagccg attcattaat gcaggttaac
    11281 ctggcttatc gaaattaata cgactcacta tagggagacc ggcagatctg atccctactc
    11341 caaaaatgtc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
    11401 ggtaatttcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag
    11461 gacagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggctat
    11521 cattcaagat gcctctgccg acagtggtcc caaagatgga ccccca
//
