LOCUS       sequence-166688-       11132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA159  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11132)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM159
FEATURES             Location/Qualifiers
     source          1..11132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     89..108
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        89..107
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        176..521
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     464..484
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    682..858
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1420..1438)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1421..1438)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2077..2096
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2199..2204,2205..2221,2222..2227))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2502..2882
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2888..3439
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3449..3701
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    3787..3811
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4236..5030
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5117..5705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5859..5876
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5891..6031
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5957..5976)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6117..6139
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6375..6569
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6635..7708)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8137..8766)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10067..10091
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10279..10301
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10293..10310
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10294..10310
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10335..11042)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 acaaaaaaaa agcccgctca ttaggcgggt taaattcccg ggtactgcag ccgattcatt
       61 aatgcaggtt aacctggctt atcgaaatta atacgactca ctatagggag accggcagat
      121 ctgatcccta ctccaaaaat gtcaaagata cagtctcaga agaccaaagg gctattgaga
      181 cttttcaaca aagggtaatt tcgggaaacc tcctcggatt ccattgccca gctatctgtc
      241 acttcatcga aaggacagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
      301 aaggaaaggc tatcattcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
      361 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
      421 gatgtgacat ctccactgac ataagggatg acgcacaacc ccactatcct tcgcaagacc
      481 cttcctctat ataaggaagt tcatttcatt tggagaggac agcccaagct tcagagctcc
      541 ctctatcaat ccaaagttgt tgttgttatg gtctaattta aatatggtct aaagaagaag
      601 aattagagct ccctctatca atccaaggaa ttcggtacgc tgaaatcacc agtctctctc
      661 tacaaatcta tctctctcta ttttctccat aaataatgtg tgagtagttt cccgataagg
      721 gaaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
      781 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
      841 agtattaaaa tccagatctc ctaaagtccc tatagatctt tgtcgtgaat ataaaccaga
      901 cacgagacga ctagacctgg agcccagacg ccgttcgaag ctagaagtac cgcttaggcg
      961 ggaggccgtt agggaaaaga tgctaaggca gggctggtta cgttgactcc cccgtaggtt
     1021 tggtttaaat atgatgaagt ggacggaagg aaggaggaag acaaggaagg ataaggttgc
     1081 aggccctgtg caaggtaaga agatggaaat ttgatagagg tacgctacta tacttatact
     1141 atacgctaag ggaatgattg tatttatacc ctataccccc taataacccc ttatcaattt
     1201 aagaaataat ccgcataagc ccccgcttaa aaattggtat cagagccatg aataggtcta
     1261 tgaccaaaac tcaagaggat aaaacctcac caaaatacga aagagtcctt aactctaaag
     1321 ataaaagatc tttcaagatc aaaactagtt ccctcacacc ggtgacgggg atcgcatgcg
     1381 acctctagac catcacattg gagcttcagc tgctcgagtt ctatagtgtc acctaaatcg
     1441 tatgtgtatg atacataagg ttatgtatta attgtagccg cgttcttacg tcgacgtacc
     1501 aattacgccc cgccctgtca ctcatcgcag tactgttgta attcattaag cattctgccg
     1561 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     1621 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtc gtccatattg
     1681 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata
     1741 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     1801 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac
     1861 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tattaccagc
     1921 tcaccgtctt tcattgccat acggaactcc ggatgagcat tcatcaggcg ggcaagaatg
     1981 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     2041 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     2101 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttctttctcc
     2161 attttagctt ccttagctcc tgaaaatctc gataacccat taaagcttat cgatgataag
     2221 ctgtcaaaca tgagaattta gcccgcctaa tgggcgggct ttttgttgtg atccgcgttg
     2281 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2341 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc ctggaagctc
     2401 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttcttaa
     2461 ttaactctct agactcacct aggatccatg aattctgaga tttttcaaat cagtgcgcaa
     2521 gacgtgacgt aagtatccga gtcagttttt atttttctac taatttggtc gtttatttcg
     2581 gcgtgtagga catggcaacc gggcctgaat ttcgcgggta ttctgtttct attccaactt
     2641 tttcttgatc cgcagccatt aacgactttt gaatagatac gctgacacgc caagcctcgc
     2701 tagtcaaaag tgtaccaaac aacgctttac agcaagaacg gaatgcgcgt gacgctcgcg
     2761 gtgacgccat ttcgcctttt cagaaatgga taaatagcct tgcttcctat tatatcttcc
     2821 caaattacca atacattaca ctagcatctg aatttcataa ccaatctcga tacaccaaat
     2881 cgaatcgatg agcccagaac gacgcccggc cgacatccgc cgtgccaccg aggcggacat
     2941 gccggcggtc tgcaccatcg tcaaccacta catcgagaca agcacggtca acttccgtac
     3001 cgagccgcag gaaccgcagg agtggacgga cgacctcgtc cgtctgcggg agcgctatcc
     3061 ctggctcgtc gccgaggtgg acggcgaggt cgccggcatc gcctacgcgg gcccctggaa
     3121 ggcacgcaac gcctacgact ggacggccga gtcgaccgtg tacgtctccc cccgccacca
     3181 gcggacggga ctgggctcca cgctctacac ccacctgctg aagtccctgg aggcacaggg
     3241 cttcaagagc gtggtcgctg tcatcgggct gcccaacgac ccgagcgtgc gcatgcacga
     3301 ggcgctcgga tatgcccccc gcggcatgct gcgggcggcc ggcttcaagc acgggaactg
     3361 gcatgacgtg ggtttctggc agctggactt cagcctgccg gtaccgcccc gtccggtcct
     3421 gcccgtcacc gagatttgac tgcagaatct tggactccca tgttggcaaa ggcaaccaaa
     3481 caaacaatga atgatccgct cctgcatatg gggcggtttg agtatttcaa ctgccatttg
     3541 ggctgaattg aagacatgct cctgtcagaa attccgtgat cttactcaat attcagtaat
     3601 ctcggccaat atcctaaatg tgcgtggctt tatctgtctt tgtattgttt catcaattca
     3661 tgtaacgttt gcttttctta tgaattttca aataaattat cagatccgag ctcgaattaa
     3721 ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt
     3781 caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca
     3841 gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg
     3901 gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc
     3961 aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa
     4021 cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact
     4081 atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt
     4141 tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc
     4201 tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg
     4261 aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg
     4321 ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc
     4381 ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag
     4441 gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc
     4501 tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg
     4561 aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg
     4621 attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata
     4681 acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc
     4741 gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg
     4801 gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc
     4861 ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca
     4921 gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc
     4981 ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat
     5041 gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5101 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5161 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5221 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     5281 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5341 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5401 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5461 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5521 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5581 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5641 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5701 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5761 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     5821 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     5881 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     5941 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     6001 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     6061 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     6121 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt
     6181 gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc
     6241 ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg
     6301 cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc
     6361 gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag
     6421 agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc
     6481 ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct
     6541 ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg
     6601 ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct
     6661 cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca
     6721 aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct
     6781 tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg
     6841 ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca
     6901 aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt
     6961 acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga
     7021 tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg
     7081 ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca
     7141 ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt
     7201 gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt
     7261 cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg
     7321 ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag
     7381 ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc
     7441 gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg
     7501 gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat
     7561 cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg
     7621 cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta
     7681 ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc
     7741 attgcagggc cggcaggcaa cccagccgct tacgcctggc caaccgcccg ttcctccaca
     7801 catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt
     7861 agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga
     7921 tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct
     7981 tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca
     8041 ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt
     8101 ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc
     8161 agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt
     8221 tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat
     8281 gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat
     8341 cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt
     8401 aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat
     8461 cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc
     8521 gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg
     8581 gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact
     8641 gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc
     8701 tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac
     8761 cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc
     8821 gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt
     8881 tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg
     8941 atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg
     9001 atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc
     9061 ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg
     9121 tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc
     9181 tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt
     9241 tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga
     9301 gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg
     9361 tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca
     9421 tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc
     9481 cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt
     9541 ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct
     9601 ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg
     9661 cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc
     9721 ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag
     9781 tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg
     9841 cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg
     9901 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc
     9961 gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg
    10021 cccttttaaa tatccgatta ttctaataaa cgctcttttc tcttaggttt acccgccaat
    10081 atatcctgtc aaacactgat agtttaaact gaaggcggga aacgacaatc tgatccaagc
    10141 tcaagctgct ctagcattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
    10201 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
    10261 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagcttg
    10321 catgcctgca ggtcctgctg agcctcgaca tgttgtcgca aaattcgccc tggacccgcc
    10381 caacgatttg tcgtcactgt caaggtttga cctgcacttc atttggggcc cacatacacc
    10441 aaaaaaatgc tgcataattc tcggggcagc aagtcggtta cccggccgcc gtgctggacc
    10501 gggttgaatg gtgcccgtaa ctttcggtag agcggacggc caatactcaa cttcaaggaa
    10561 tctcacccat gcgcgccggc ggggaaccgg agttcccttc agtgagcgtt attagttcgc
    10621 cgctcggtgt gtcgtagata ctagcccctg gggcactttt gaaatttgaa taagatttat
    10681 gtaatcagtc ttttaggttt gaccggttct gccgcttttt ttaaaattgg atttgtaata
    10741 ataaaacgca attgtttgtt attgtggcgc tctatcatag atgtcgctat aaacctattc
    10801 agcacaatat attgttttca ttttaatatt gtacatataa gtagtagggt acaatcagta
    10861 aattgaacgg agaatattat tcataaaaat acgatagtaa cgggtgatat attcattaga
    10921 atgaaccgaa accggcggta aggatctgag ctacacatgc tcaggttttt tacaacgtgc
    10981 acaacagaat tgaaagcaaa tatcatgcga tcataggcgt ctcgcatatc tcattaaagc
    11041 aggactctag ggactagtcc cgggtcttaa ttaagtttgc aagcagcaga ttacgcgcag
    11101 aaaaaaagga tctcaaggag atcctttgga tc
//
