LOCUS       sequence-166691-       12903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA160  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12903)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 12903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM160
FEATURES             Location/Qualifiers
     source          1..12903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    507..683
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1245..1263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1246..1263)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1902..1921
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2024..2029,2030..2046,2047..2052))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      complement(2330..2582)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2617..2634)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(2641..4458,4649..4663))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          4459..4648
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(4682..4728)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(4817..4834)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4817..4833)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4826..4848)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5036..5060
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6361..6990
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7419..8492
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8558..8752
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8988..9010)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9096..9236
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9151..9170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9251..9268)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9422..10010)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10097..10891)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11316..11340
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(11426..11678)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(11688..12239)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(12245..12625)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     primer_bind     12817..12836
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12817..12835
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagctttggc
      361 atacaggcta gagccaggca gttgttgttg ttatggtcta atttaaatat ggtctaaaga
      421 agaagaattg gcatacaggc tagagccagg cagaattccg tacgctgaaa tcaccagtct
      481 ctctctacaa atctatctct ctctattttc tccataaata atgtgtgagt agtttcccga
      541 taagggaaat tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt
      601 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaagccaa
      661 aatccagtac taaaatccag atctcctaaa gtccctatag atctttgtcg tgaatataaa
      721 ccagacacga gacgactaga cctggagccc aggcgccgtt cgaagctaga agtaccgctt
      781 aggcaggagg ccgttaggga aaagatgcta aggcagggtt ggttacgttg actcccccgt
      841 aggtttggtt taaatatgat gaagtggacg gaaggaagga ggaagacaag gaaggataag
      901 gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat agaggtacgc tactatactt
      961 atactatacg ctaagggaat gattgtattt ataccctata ccccctaata accccttatc
     1021 aatttaagaa ataatccgca taagcccccg cttaaaaatt ggtatcagag ccatgaatag
     1081 gtctatgacc aaaactcaag aggataaaac ctcaccaaaa tacgaaagag tccttaactc
     1141 taaagataaa agatctttca agatcaaaac tagttccctc acaccggtga cggggatcgc
     1201 atgcgacctc tagaccatca cattggagct tcagctgctc gagttctata gtgtcaccta
     1261 aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc ttacgtcgac
     1321 gtaccaatta cgccccgccc tgtcactcat cgcagtactg ttgtaattca ttaagcattc
     1381 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1441 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     1501 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     1561 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     1621 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     1681 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatta
     1741 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
     1801 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacagtc tttaaaaagg
     1861 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     1921 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttctt
     1981 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcattaaag cttatcgatg
     2041 ataagctgtc aaacatgaga atttagcccg cctaatgagc gggctttttg ttgtgatccg
     2101 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2161 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt cccccctgga
     2221 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2281 cttaattaac cgccaatata tcctgtcaaa cactgatagt ttaattcccg atctagtaac
     2341 atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc
     2401 gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc
     2461 atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc
     2521 atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga
     2581 tcggggaaat tcgagctggt cacctgtaat tcacacgtgg tggtggtggt ggtggctagc
     2641 ttgtttgcct ccctgctgcg gtttttcacc gaagttcatg ccagtccagc gtttttgcag
     2701 cagaaaagcc gccgacttcg gtttgcggtc gcgagtgaag atccctttct tgttaccgcc
     2761 aacgcgcaat atgccttgcg aggtcgcaaa atcggcgaaa ttccatacct gttcaccgac
     2821 gacggcgctg acgcgatcaa agacgcggtg atacatatcc agccatgcac actgatactc
     2881 ttcactccac atgtcggtgt acattgagtg cagcccggct aacgtatcca cgccgtattc
     2941 ggtgatgata atcggctgat gcagtttctc ctgccaggcc agaagttctt tttccagtac
     3001 cttctctgcc gtttccaaat cgccgctttg gacataccat ccgtaataac ggttcaggca
     3061 cagcacatca aagagatcgc tgatggtatc ggtgtgagcg tcgcagaaca ttacattgac
     3121 gcaggtgatc ggacgcgtcg ggtcgagttt acgcgttgct tccgccagtg gcgcgaaata
     3181 ttcccgtgca ccttgcggac gggtatccgg ttcgttggca atactccaca tcaccacgct
     3241 tgggtggttt ttgtcacgcg ctatcagctc tttaatcgcc tgtaagtgcg cttgctgagt
     3301 ttccccgttg actgcctctt cgctgtacag ttctttcggc ttgttgcccg cttcgaaacc
     3361 aatgcctaaa gagaggttaa agccgacagc agcagtttca tcaatcacca cgatgccatg
     3421 ttcatctgcc cagtcgagca tctcttcagc gtaagggtaa tgcgaggtac ggtaggagtt
     3481 ggccccaatc cagtccatta atgcgtggtc gtgcaccatc agcacgttat cgaatccttt
     3541 gccacgcaag tccgcatctt catgacgacc aaagccagta aagtagaacg gtttgtggtt
     3601 aatcaggaac tgttcgccct tcactgccac tgaccggatg ccgacgcgaa gcgggtagat
     3661 atcacactct gtctggcttt tggctgtgac gcacagttca tagagataac cttcacccgg
     3721 ttgccagagg tgcggattca ccacttgcaa agtcccgcta gtgccttgtc cagttgcaac
     3781 cacctgttga tccgcatcac gcagttcaac gctgacatca ccattggcca ccacctgcca
     3841 gtcaacagac gcgtggttac agtcttgcgc gacatgcgtc accacggtga tatcgtccac
     3901 ccaggtgttc ggcgtggtgt agagcattac gctgcgatgg attccggcat agttaaagaa
     3961 atcatggaag taagactgct ttttcttgcc gttttcgtcg gtaatcacca ttcccggcgg
     4021 gatagtctgc cagttcagtt cgttgttcac acaaacggtg atacgtacac ttttcccggc
     4081 aataacatac ggcgtgacat cggcttcaaa tggcgtatag ccgccctgat gctccatcac
     4141 ttcctgatta ttgacccaca ctttgccgta atgagtgacc gcatcgaaac gcagcacgat
     4201 acgctggcct gcccaacctt tcggtataaa gacttcgcgc tgataccaga cgttgcccgc
     4261 ataattacga atatctgcat cggcgaactg atcgttaaaa ctgcctggca cagcaattgc
     4321 ccggctttct tgtaacgcgc tttcccacca acgctgatca attccacagt tttcgcgatc
     4381 cagactgaat gcccacaggc cgtcgagttt tttgatttca cgggttgggg tttctacagg
     4441 acggacgagt cgtcggttct gtaactatca tcatcatcat agacacacga aataaagtaa
     4501 tcagattatc agttaaagct atgtaatatt tacaccataa ccaatcaatt aaaaaataga
     4561 tcagtttaaa gaaagatcaa agctcaaaaa aataaaaaga gaaaagggtc ctaaccaaga
     4621 aaatgaagga gaaaaactag aaatttaccc tcagatctac catggtcaag agtcccccgt
     4681 gttctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga gagacaaaag
     4741 ggagacaaaa gggagacaaa agggagacaa aagggagaca aaagggagac aaaagggaga
     4801 caaaaggaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     4861 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     4921 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca
     4981 gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca
     5041 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta
     5101 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
     5161 cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct
     5221 gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac
     5281 aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt
     5341 agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc
     5401 tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac
     5461 tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc
     5521 gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca
     5581 ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg
     5641 aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg
     5701 gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg
     5761 accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc
     5821 ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca
     5881 ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg
     5941 agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg
     6001 cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat
     6061 gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga
     6121 gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca
     6181 tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc
     6241 tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg
     6301 tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc
     6361 atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc
     6421 catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc
     6481 cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc
     6541 atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg
     6601 atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc
     6661 gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag
     6721 ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg
     6781 cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag
     6841 ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc
     6901 ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg
     6961 gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa
     7021 gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg
     7081 ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc
     7141 acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg
     7201 aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta
     7261 gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc
     7321 cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc
     7381 tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg
     7441 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     7501 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     7561 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     7621 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     7681 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     7741 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     7801 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     7861 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     7921 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     7981 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     8041 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     8101 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     8161 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     8221 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     8281 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     8341 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     8401 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     8461 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     8521 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga
     8581 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
     8641 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
     8701 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
     8761 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
     8821 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
     8881 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
     8941 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     9001 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     9061 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     9121 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     9181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     9241 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9301 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9361 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9421 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9481 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9541 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9601 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9661 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9721 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9781 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9841 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     9901 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9961 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    10021 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    10081 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
    10141 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
    10201 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
    10261 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
    10321 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
    10381 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
    10441 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
    10501 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
    10561 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
    10621 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
    10681 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
    10741 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
    10801 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
    10861 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
    10921 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
    10981 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
    11041 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
    11101 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
    11161 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
    11221 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
    11281 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
    11341 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac
    11401 gccgaattaa ttcgagctcg gatctgataa tttatttgaa aattcataag aaaagcaaac
    11461 gttacatgaa ttgatgaaac aatacaaaga cagataaagc cacgcacatt taggatattg
    11521 gccgagatta ctgaatattg agtaagatca cggaatttct gacaggagca tgtcttcaat
    11581 tcagcccaaa tggcagttga aatactcaaa ccgccccata tgcaggagcg gatcattcat
    11641 tgtttgtttg gttgcctttg ccaacatggg agtccaagat tctgcagtca aatctcggtg
    11701 acgggcagga ccggacgggg cggtaccggc aggctgaagt ccagctgcca gaaacccacg
    11761 tcatgccagt tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg ggcatatccg
    11821 agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc
    11881 ttgaagccct gtgcctccag ggacttcagc aggtgggtgt agagcgtgga gcccagtccc
    11941 gtccgctggt ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg
    12001 cgtgccttcc aggggcccgc gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg
    12061 agccagggat agcgctcccg cagacggacg aggtcgtccg tccactcctg cggttcctgc
    12121 ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc
    12181 gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc tgggctcatc
    12241 gattcgattt ggtgtatcga gattggttat gaaattcaga tgctagtgta atgtattggt
    12301 aatttgggaa gatataatag gaagcaaggc tatttatcca tttctgaaaa ggcgaaatgg
    12361 cgtcaccgcg agcgtcacgc gcattccgtt cttgctgtaa agcgttgttt ggtacacttt
    12421 tgactagcga ggcttggcgt gtcagcgtat ctattcaaaa gtcgttaatg gctgcggatc
    12481 aagaaaaagt tggaatagaa acagaatacc cgcgaaattc aggcccggtt gccatgtcct
    12541 acacgccgaa ataaacgacc aaattagtag aaaaataaaa actgactcgg atacttacgt
    12601 cacgtcttgc gcactgattt gaaaaatctc agaattcatg gatcctaggt gagtctagag
    12661 agttaattaa gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aaggagatcc
    12721 tttggatcac aaaaaaaaag cccgctcatt aggcgggtta aattcccggg tactgcagcc
    12781 gattcattaa tgcaggttaa cctggcttat cgaaattaat acgactcact atagggagac
    12841 cggcagatct gatccctact ccaaaaatgt caaagataca gtctcagaag accaaagggc
    12901 tat
//
