LOCUS       sequence-166694-       11602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA161.1  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM161.1
FEATURES             Location/Qualifiers
     source          1..11602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(429..448)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(430..448)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      627..1334
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(1359..1376)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1359..1375)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1368..1390)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    1578..1602
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2903..3532
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3961..5034
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5100..5294
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5530..5552)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5638..5778
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5693..5712
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5793..5810)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5964..6552)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6639..7433)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7858..7882
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(7968..8220)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(8230..8781)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(8787..9167)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        join(9573..9578,9579..9595,9596..9601)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(9704..9723)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        10362..10380
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     10362..10379
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    10942..11118
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(join(11279..11602,1..22))
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(11316..11336)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 taccctttgt tgaaaagtct caatagccct ttggtcttct gagacagtat ctttgacatt
       61 tttggagtag gggtgggatt gtgcgtcatc ccttacgtca gtggagatgt cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttcttttccc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttga atgatagcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact gtcctttcga tgaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tcccgaaatt accctttgtt gaaaagtctc
      361 aatagccctt tggtcttctg agactgtatc tttgacattt ttggagtagg gatcagatct
      421 gccggtctcc ctatagtgag tcgtattaat ttcgataagc caggttaacc tgcattaatg
      481 aatcggctgc agtacccggg aatttaaccc gcctaatgag cgggcttttt ttttgtgatc
      541 caaaggatct ccttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacttaatt
      601 aagacccggg actagtccct agagtcctgc tttaatgaga tatgcgagac gcctatgatc
      661 gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct
      721 cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat
      781 ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa
      841 tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc
      901 gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag
      961 aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca
     1021 ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc
     1081 ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct
     1141 accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc
     1201 tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc
     1261 aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg
     1321 tcgaggctca gcaggacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt
     1381 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     1441 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     1501 ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag
     1561 tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt
     1621 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
     1681 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc
     1741 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg
     1801 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
     1861 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
     1921 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
     1981 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
     2041 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
     2101 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
     2161 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
     2221 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
     2281 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
     2341 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
     2401 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
     2461 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
     2521 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
     2581 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
     2641 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
     2701 acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
     2761 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
     2821 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
     2881 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
     2941 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
     3001 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
     3061 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
     3121 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
     3181 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
     3241 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
     3301 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
     3361 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
     3421 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
     3481 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
     3541 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
     3601 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
     3661 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
     3721 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
     3781 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
     3841 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
     3901 cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
     3961 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
     4021 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
     4081 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
     4141 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
     4201 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
     4261 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
     4321 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
     4381 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
     4441 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
     4501 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
     4561 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
     4621 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
     4681 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
     4741 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
     4801 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
     4861 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
     4921 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
     4981 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
     5041 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct
     5101 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
     5161 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
     5221 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
     5281 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
     5341 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
     5401 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
     5461 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
     5521 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     5581 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     5641 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
     5701 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     5761 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5821 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5881 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5941 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6001 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6061 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6121 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6181 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6241 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6301 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6361 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     6421 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6481 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6541 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6601 aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa
     6661 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
     6721 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
     6781 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
     6841 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
     6901 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
     6961 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
     7021 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
     7081 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
     7141 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
     7201 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
     7261 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
     7321 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
     7381 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
     7441 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
     7501 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
     7561 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
     7621 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
     7681 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
     7741 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
     7801 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
     7861 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac
     7921 gtttttaatg tactgaatta acgccgaatt aattcgagct cggatctgat aatttatttg
     7981 aaaattcata agaaaagcaa acgttacatg aattgatgaa acaatacaaa gacagataaa
     8041 gccacgcaca tttaggatat tggccgagat tactgaatat tgagtaagat cacggaattt
     8101 ctgacaggag catgtcttca attcagccca aatggcagtt gaaatactca aaccgcccca
     8161 tatgcaggag cggatcattc attgtttgtt tggttgcctt tgccaacatg ggagtccaag
     8221 attctgcagt caaatctcgg tgacgggcag gaccggacgg ggcggtaccg gcaggctgaa
     8281 gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag
     8341 catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag
     8401 cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt
     8461 gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg gagacgtaca cggtcgactc
     8521 ggccgtccag tcgtaggcgt tgcgtgcctt ccaggggccc gcgtaggcga tgccggcgac
     8581 ctcgccgtcc acctcggcga cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc
     8641 cgtccactcc tgcggttcct gcggctcggt acggaagttg accgtgcttg tctcgatgta
     8701 gtggttgacg atggtgcaga ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc
     8761 cgggcgtcgt tctgggctca tcgattcgat ttggtgtatc gagattggtt atgaaattca
     8821 gatgctagtg taatgtattg gtaatttggg aagatataat aggaagcaag gctatttatc
     8881 catttctgaa aaggcgaaat ggcgtcaccg cgagcgtcac gcgcattccg ttcttgctgt
     8941 aaagcgttgt ttggtacact tttgactagc gaggcttggc gtgtcagcgt atctattcaa
     9001 aagtcgttaa tggctgcgga tcaagaaaaa gttggaatag aaacagaata cccgcgaaat
     9061 tcaggcccgg ttgccatgtc ctacacgccg aaataaacga ccaaattagt agaaaaataa
     9121 aaactgactc ggatacttac gtcacgtctt gcgcactgat ttgaaaaatc tcagaattca
     9181 tggatcctag gtgagtctag agagttatcc cttaattaag aaaggcggac aggtatccgg
     9241 gaatttaacc cgcctaatga gcgggctttt tttttgtgat ccagaggatc tccttgagat
     9301 cctttttttc tgcgcgtaat ctgctgcttg caaacttaat taagaaaggc ggacaggtat
     9361 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg ggggaaacgc
     9421 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9481 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggatc acaacaaaaa
     9541 gcccgctcat taggcgggct aaattctcat gtttgacagc ttatcatcga taagctttaa
     9601 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaagaaa tcactggata
     9661 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     9721 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     9781 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gtctgatgaa
     9841 tgctcatccg gagttccgta tggcaatgaa agacggtgag ctggtaatat gggatagtgt
     9901 tcacccttgt tacaccgttt tccatgagca aaccgaaacg ttttcatcgc tctggagtga
     9961 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
    10021 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
    10081 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
    10141 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
    10201 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
    10261 acaacagtac tgcgatgagt gacagggcgg ggcgtaattg gtacgtcgac gtaagaacgc
    10321 ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg acactataga
    10381 actcgagcag ctgaagctcc aatgtgatgg tctagaggtc gcatgcgatc cccgtcaccg
    10441 gtgtgaggga actagttttg atcttgaaag atcttttatc tttagagtta aggacccttt
    10501 cgcattttgg tgaggtttta tcctcttgag ttttggtcat agacctattc atggctctga
    10561 taccaatttt taagcggggg cttatgcgga ttatttctta aattgataag gggttattag
    10621 ggggtatagg gtataaatac aatcgttccc ttagcgtata gtataagtat agtagcgtac
    10681 ctctatcaaa tttccatctt cttaccttgc acggggcctg caaccttatc cttccttgtc
    10741 ttcctccttc cttccgtcca cttcatcata tttaaaccaa acctacgggg gagtcaacgt
    10801 aaccaaccct gccttagcat cttttcccta acggcctcct gcctaagcgg tacttctagc
    10861 ttcgaacggc gtctgggctc caggtctagt cgtctcgtgt ctggtttata ttcacgacaa
    10921 agatctatag ggactttagg agatctggat tttagtactg gatttcggtt ttaggaatta
    10981 gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg
    11041 ctcaacacat gagcgaaacc ctataagaac cctaatttcc cttatcggga aactactcac
    11101 acattattta tggagaaaat agagagagat agatttgtag agagagactg gtgatttcag
    11161 cgtaccgaat tctgaaagtg acatgtacat cggggtattc ttcttcttta gaccatattt
    11221 aaattagacc ataacaacaa caactgaaag tgacatgtac atcggggtaa gcttgggctg
    11281 tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg
    11341 gttgtgcgtc atcccttatg tcagtggaga tgtcacatca atccacttgc tttgaagacg
    11401 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tttttgggac
    11461 cactgtcggc agaggcatct tgaatgatag cctttccttt atcgcaatga tggcatttgt
    11521 aggagccacc tcccttttct actgtccttt cgatgaggtg acagatagct gggcaatgga
    11581 atccgaggag gtttcccgaa at
//
