LOCUS       sequence-166707-        5529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing DNMT3A , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_DNMT3A_p.G746V
FEATURES             Location/Qualifiers
     source          1..5529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             189..2927
                     /codon_start=1
                     /product="DNA methyltransferase 3A"
                     /label=DNMT3A
                     /translation="MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTTA
                     RKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPEEG
                     SPAGGQKGGAPAEGEGAAETLPEASRAVENGCCTPKEGRGAPAEAGKEQKETNIESMKM
                     EGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIA
                     GMNAVEENQGPGESQKVEEASPPAVQQPTDPASPTVATTPEPVGSDAGDKNATKAGDDE
                     PEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVC
                     VEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPVCHDSDESDTAKAV
                     EVQNKPMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPR
                     KSTAEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFVGGMCQNC
                     KNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAI
                     KEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIR
                     VLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKH
                     IQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEVDDRPFFW
                     LFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDK
                     LELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFP
                     VHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV"
     protein_bind    complement(2929..3028)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3045..3064)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3046..3064)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3069..3085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3069..3085)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3516..4307
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4400..4988
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4889..4908
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5142..5159
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5318..5345)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5437..5523)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gcccgccatg ccctccagcg gccccgggga caccagcagc tctgctgcgg
      241 agcgggagga ggaccgaaag gacggagagg agcaggagga gccgcgtggc aaggaggagc
      301 gccaagagcc cagcaccacg gcacggaagg tggggcggcc tgggaggaag cgcaagcacc
      361 ccccggtgga aagcggtgac acgccaaagg accctgcggt gatctccaag tccccatcca
      421 tggcccagga ctcaggcgcc tcagagctat tacccaatgg ggacttggag aagcggagtg
      481 agccccagcc agaggagggg agccctgctg gggggcagaa gggcggggcc ccagcagagg
      541 gagagggtgc agctgagacc ctgcctgaag cctcaagagc agtggaaaat ggctgctgca
      601 cccccaagga gggccgagga gcccctgcag aagcgggcaa agaacagaag gagaccaaca
      661 tcgaatccat gaaaatggag ggctcccggg gccggctgcg gggtggcttg ggctgggagt
      721 ccagcctccg tcagcggccc atgccgaggc tcaccttcca ggcgggggac ccctactaca
      781 tcagcaagcg caagcgggac gagtggctgg cacgctggaa aagggaggct gagaagaaag
      841 ccaaggtcat tgcaggaatg aatgctgtgg aagaaaacca ggggcccggg gagtctcaga
      901 aggtggagga ggccagccct cctgctgtgc agcagcccac tgaccccgca tcccccactg
      961 tggctaccac gcctgagccc gtggggtccg atgctgggga caagaatgcc accaaagcag
     1021 gcgatgacga gccagagtac gaggacggcc ggggctttgg cattggggag ctggtgtggg
     1081 ggaaactgcg gggcttctcc tggtggccag gccgcattgt gtcttggtgg atgacgggcc
     1141 ggagccgagc agctgaaggc acccgctggg tcatgtggtt cggagacggc aaattctcag
     1201 tggtgtgtgt tgagaagctg atgccgctga gctcgttttg cagtgcgttc caccaggcca
     1261 cgtacaacaa gcagcccatg taccgcaaag ccatctacga ggtcctgcag gtggccagca
     1321 gccgcgcggg gaagctgttc ccggtgtgcc acgacagcga tgagagtgac actgccaagg
     1381 ccgtggaggt gcagaacaag cccatgattg aatgggccct ggggggcttc cagccttctg
     1441 gccctaaggg cctggagcca ccagaagaag agaagaatcc ctacaaagaa gtgtacacgg
     1501 acatgtgggt ggaacctgag gcagctgcct acgcaccacc tccaccagcc aaaaagcccc
     1561 ggaagagcac agcggagaag cccaaggtca aggagattat tgatgagcgc acaagagagc
     1621 ggctggtgta cgaggtgcgg cagaagtgcc ggaacattga ggacatctgc atctcctgtg
     1681 ggagcctcaa tgttaccctg gaacaccccc tcttcgttgg aggaatgtgc caaaactgca
     1741 agaactgctt tctggagtgt gcgtaccagt acgacgacga cggctaccag tcctactgca
     1801 ccatctgctg tgggggccgt gaggtgctca tgtgcggaaa caacaactgc tgcaggtgct
     1861 tttgcgtgga gtgtgtggac ctcttggtgg ggccgggggc tgcccaggca gccattaagg
     1921 aagacccctg gaactgctac atgtgcgggc acaagggtac ctacgggctg ctgcggcggc
     1981 gagaggactg gccctcccgg ctccagatgt tcttcgctaa taaccacgac caggaatttg
     2041 accctccaaa ggtttaccca cctgtcccag ctgagaagag gaagcccatc cgggtgctgt
     2101 ctctctttga tggaatcgct acagggctcc tggtgctgaa ggacttgggc attcaggtgg
     2161 accgctacat tgcctcggag gtgtgtgagg actccatcac ggtgggcatg gtgcggcacc
     2221 aggggaagat catgtacgtc ggggacgtcc gcagcgtcac acagaagcat atccaggagt
     2281 ggggcccatt cgatctggtg attgggggca gtccctgcaa tgacctctcc atcgtcaacc
     2341 ctgctcgcaa gggcctctac gagggcactg gccggctctt ctttgagttc taccgcctcc
     2401 tgcatgatgc gcggcccaag gaggtagatg atcgcccctt cttctggctc tttgagaatg
     2461 tggtggccat gggcgttagt gacaagaggg acatctcgcg atttctcgag tccaaccctg
     2521 tgatgattga tgccaaagaa gtgtcagctg cacacagggc ccgctacttc tggggtaacc
     2581 ttcccggtat gaacaggccg ttggcatcca ctgtgaatga taagctggag ctgcaggagt
     2641 gtctggagca tggcaggata gccaagttca gcaaagtgag gaccattact acgaggtcaa
     2701 actccataaa gcagggcaaa gaccagcatt ttcctgtctt catgaatgag aaagaggaca
     2761 tcttatggtg cactgaaatg gaaagggtat ttggtttccc agtccactat actgacgtct
     2821 ccaacatgag ccgcttggcg aggcagagac tgctgggccg gtcatggagc gtgccagtca
     2881 tccgccacct cttcgctccg ctgaaggagt attttgcgtg tgtgtgattg ccaactttct
     2941 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     3001 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
     3061 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     3121 acattgcaca agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg
     3181 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     3241 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     3301 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     3361 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     3421 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     3481 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     3541 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     3601 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     3661 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     3721 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     3781 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     3841 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     3901 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     3961 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     4021 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     4081 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     4141 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     4201 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     4261 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc
     4321 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     4381 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4441 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4501 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     4561 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4621 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4681 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4741 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     4801 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4861 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     4921 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     4981 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5041 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5101 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5161 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     5221 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     5281 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     5341 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     5401 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     5461 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     5521 ttgatgcct
//
