LOCUS       sequence-16672-e        1371 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3136
FEATURES             Location/Qualifiers
     source          1..1371
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             409..429
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(472..500)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gatcttacta actaactatt ctcatttaaa ttttcagagc ttaaaaatgg ctgaaatcac
       61 tcacaacgat ggatacgcta acaacttgga aatgaaataa gcttgcatgc ctgcaggcct
      121 tggtcgactc tagacacttt tcagctacct agaattacaa ttggatatcc ccgcctccca
      181 atccacccac ccagggaaaa agaagggctc gccgaaaatc aaagttatct ccaggctcgc
      241 gcatcccacc gagcggttga cttctctcca ccacttttca ttttaaccct cggggtacgg
      301 gattggccaa aggacccaaa ggtatgtttc gaatgatact aacataacat agaacatttt
      361 caggaggacc cttcttggag ggtaccgagc tcagaaaaaa tgactgctcc aaagaagaag
      421 cgtaaggtac cggtagaaaa aatgagtaaa ggagaagaac ttttcactgg agttgtccca
      481 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      541 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      601 cctgttccat gggtaagttt aaacatatat atactaacta accctgatta tttaaatttt
      661 cagccaacac ttgtcactac tttctgttat ggtgttcaat gcttctcgag atacccagat
      721 catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
      781 actatatttt tcaaagatga cgggaactac aagacacgta agtttaaaca gttcggtact
      841 aactaaccat acatatttaa attttcaggt gctgaagtca agtttgangg tgatacccct
      901 tgttaataga atcgagtnaa aaggtattga ttttaaagaa gatggaaaca ttcttgganc
      961 naattggaat acaactataa ctcaccnatg tntacatcnt ggcanacaaa ccaaagangg
     1021 natccnagtt gtagtttaac ntgatttnnc aactactatc tgattaaatt ttcanacntc
     1081 caaattnaac ccncattgaa aagggagcgt tcactngcca aacctttntc aanaannnnc
     1141 cattgggang gcctgtcttt tacaaaaaca ttaccgnccn nantntgccn ttnaaaatcc
     1201 aannaaaaaa aacccnggnc ctttgatnta accgggngga nacccggngg aaannnaaag
     1261 gcngggggga aanaaaaccc nnaatggccg attncccgtt ncccnnnggg gaaanccntt
     1321 tnangggggg naacggggnc aantccnggn cccntccgng naaaaangcc c
//
