LOCUS       sequence-166732-        4362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ZNF454 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ZNF454_p.H462Y
FEATURES             Location/Qualifiers
     source          1..4362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2241..2340)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2357..2376)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2358..2376)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2381..2397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2381..2397)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2828..3619
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVYDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3712..4300
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4201..4220
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggctgtca gccacctgcc aaccatggtc caggaatcgg tgaccttcaa
      721 ggatgtggct atactgttca cccaggaaga gtgggggcag ctgagccccg cccagagggc
      781 cctgtacagg gacgtgatgc tggagaacta cagcaacctg gtctcactgg gactcttagg
      841 acccaaacca gatacgtttt cccagctaga aaaaagggaa gtgtggatgc cagaggacac
      901 ccctggaggc ttctgtcttg actggatgac tatgcctgcc agtaagaaat ctactgtcaa
      961 ggcagagatt cctgaagaag aattggatca atggacaata aaggaaagat tcagtagcag
     1021 tagtcactgg aagtgtgcta gcctgctgga gtggcaatgt ggaggccagg agatcagttt
     1081 gcagcgagtg gtactcactc accccaacac cccatcacag gaatgtgatg aatccgggag
     1141 cactatgagc tcatctcttc acagtgctca aagtcaggga tttcaaccta gcaaaaatgc
     1201 ctttgagtgt agtgagtgtg gaaaagtctt ctctaagagt tcaactctta ataaacatca
     1261 gaaaattcat aatgaaaaaa atgcaaatca gaaaattcat attaaggaga aaagatatga
     1321 atgtagagaa tgtgggaaag cctttcacca gagtacgcac cttatccatc accaaagaat
     1381 tcacactggc gagaaaccct atgaatgtaa ggaatgtggc aaggccttct cagtgagctc
     1441 ctcacttacg taccatcaga aaattcatac tggagagaag ccttttgaat gcaacttatg
     1501 tggaaaagct tttatccgaa atatacacct tgcccatcat catagaatac atactggaga
     1561 gaaacctttt aaatgtaaca tttgtgaaaa agcctttgtg tgcagggcac accttaccaa
     1621 acaccagaat atccacagtg gagagaaacc ctataaatgc aatgaatgtg gaaaagcctt
     1681 taatcagagt acaagtttcc ttcagcatca gagaattcac actggagaga aaccctttga
     1741 atgtaatgaa tgtgggaagg ccttcagggt gaactcttcc cttactgaac atcagagaat
     1801 tcatactgga gagaaacctt ataaatgtaa tgaatgtggg aaagctttca gggataattc
     1861 atcctttgca cgacatcgga aaattcacac tggagagaaa ccttacagat gtggcttgtg
     1921 tgagaaagcc tttcgggacc aatcagcact agcccaacat cagagaattc atactgggga
     1981 aaaaccttat acatgtaaca tatgtgaaaa agccttcagt gaccattcag cccttaccca
     2041 acataagaga atttatacta gggaaaaacc ttacaaatgt aaaatctgtg agaaagcctt
     2101 tatccgaagc actcacctga ctcaacatca gaggattcac acaggagaga aaccctataa
     2161 atgtaataaa tgtgggaaag cttttaacca gactgcaaac ctcattcagc atcagagaca
     2221 tcatattgga gagaagtgat tgccaacttt cttgtacaaa gttgtcatta taagaaagca
     2281 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     2341 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     2401 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     2461 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc
     2521 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc
     2581 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac
     2641 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt
     2701 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     2761 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt
     2821 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg
     2881 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt
     2941 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct
     3001 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg
     3061 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgtacgacg acatcattcc
     3121 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct
     3181 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc
     3241 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc
     3301 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga
     3361 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt
     3421 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc
     3481 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg
     3541 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac
     3601 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta
     3661 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3721 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3781 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3841 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3901 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3961 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4021 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4081 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4141 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4201 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4261 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4321 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
