LOCUS       sequence-166753-        6009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing XPO1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_XPO1_p.E571K
FEATURES             Location/Qualifiers
     source          1..6009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3409..3508)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3525..3544)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3526..3544)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3549..3565)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3549..3565)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3996..4787
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4880..5468
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5369..5388
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5622..5639
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5798..5825)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5917..6003)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgccagca attatgacaa tgttagcaga ccatgcagct cgtcagctgc
      241 ttgatttcag ccaaaaactg gatatcaact tattagataa tgtggtgaat tgcttatacc
      301 atggagaagg agcccagcaa agaatggctc aagaagtact gacacattta aaggagcatc
      361 ctgatgcttg gacaagagtc gacacaattt tggaattttc tcagaatatg aatacgaaat
      421 actatggact acaaattttg gaaaatgtga taaaaacaag gtggaagatt cttccaagga
      481 accagtgcga aggaataaaa aaatacgttg ttggcctcat tatcaagacg tcatctgacc
      541 caacttgtgt agagaaagaa aaggtgtata tcggaaaatt aaatatgatc cttgttcaga
      601 tactgaaaca agaatggccc aaacattggc caacttttat cagtgatatt gttggagcaa
      661 gtaggaccag cgaaagtctc tgtcaaaata atatggtgat tcttaaactc ttgagtgaag
      721 aagtatttga tttctctagt ggacagataa cccaagtcaa atctaagcat ttaaaagaca
      781 gcatgtgcaa tgaattctca cagatatttc aactgtgtca gtttgtaatg gaaaattctc
      841 aaaatgctcc acttgtacat gcaaccttgg aaacattgct cagatttctg aactggattc
      901 ccctgggata tatttttgag accaaattaa tcagcacatt gatttataag ttcctgaatg
      961 ttccaatgtt tcgaaatgtc tctctgaagt gcctcactga gattgctggt gtgagtgtaa
     1021 gccaatatga agaacaattt gtaacactat ttactctgac aatgatgcaa ctaaagcaga
     1081 tgcttccttt aaataccaat attcgacttg cgtactcaaa tggaaaagat gatgaacaga
     1141 acttcattca aaatctcagt ttgtttctct gcacctttct taaggaacat gatcaactta
     1201 tagaaaaaag attaaatctc agggaaactc ttatggaggc ccttcattat atgttgttgg
     1261 tatctgaagt agaagaaact gaaatcttta aaatttgtct tgaatactgg aatcatttgg
     1321 ctgctgaact ctatagagag agtccattct ctacatctgc ctctccgttg ctttctggaa
     1381 gtcaacattt tgatgttcct cccaggagac agctatattt gcccatgtta ttcaaggtcc
     1441 gtttattaat ggttagtcga atggctaaac cagaggaagt attggttgta gagaatgatc
     1501 aaggagaagt tgtgagagaa ttcatgaagg atacagattc cataaatttg tataagaata
     1561 tgagggaaac attggtttat cttactcatc tggattatgt agatacagaa agaataatga
     1621 cagagaagct tcacaatcaa gtgaatggta cagagtggtc atggaaaaat ttgaatacat
     1681 tgtgttgggc aataggctcc attagtggag caatgcatga agaggacgaa aaacgatttc
     1741 ttgttactgt tataaaggat ctattaggat tatgtgaaca gaaaagaggc aaagataata
     1801 aagctattat tgcatcaaat atcatgtaca tagtaggtca atacccacgt tttttgagag
     1861 ctcactggaa atttctgaag actgtagtta acaagctgtt caaattcatg catgagaccc
     1921 atgatggagt ccaggatatg gcttgtgata ctttcattaa aatagcccaa aaatgccgca
     1981 ggcatttcgt tcaggttcag gttggagaag tgatgccatt tattgatgaa attttgaaca
     2041 acattaacac tattatttgt gatcttcagc ctcaacaggt tcatacgttt tatgaagctg
     2101 tggggtacat gattggtgca caaacagatc aaacagtaca agaacacttg atagaaaagt
     2161 acatgttact ccctaatcaa gtgtgggata gtataatcca gcaggcaacc aaaaatgtgg
     2221 atatactgaa agatcctgaa acagtcaagc agcttggtag cattttgaaa acaaatgtga
     2281 gagcctgcaa agctgttgga cacccctttg taattcagct tggaagaatt tatttagata
     2341 tgcttaatgt atacaagtgc ctcagtgaaa atatttctgc agctatccaa gctaatggtg
     2401 aaatggttac aaagcaacca ttgattagaa gtatgcgaac tgtaaaaagg gaaactttaa
     2461 agttaatatc tggttgggtg agccgatcca atgatccaca gatggtcgct gaaaattttg
     2521 ttccccctct gttggatgca gttctcattg attatcagag aaatgtccca gctgctagag
     2581 aaccagaagt gcttagtact atggccataa ttgtcaacaa gttaggggga catataacag
     2641 ctgaaatacc tcaaatattt gatgctgttt ttgaatgcac attgaatatg ataaataagg
     2701 actttgaaga atatcctgaa catagaacga actttttctt actacttcag gctgtcaatt
     2761 ctcattgttt cccagcattc cttgctattc cacctacaca gtttaaactt gttttggatt
     2821 ccatcatttg ggctttcaaa catactatga ggaatgtcgc agatacgggc ttacagatac
     2881 tttttacact cttacaaaat gttgcacaag aagaagctgc agctcagagt ttttatcaaa
     2941 cttatttttg tgatattctc cagcatatct tttctgttgt gacagacact tcacatactg
     3001 ctggtttaac aatgcatgca tcaattcttg catatatgtt taatttggtt gaagaaggaa
     3061 aaataagtac atcattaaat cctggaaatc cagttaacaa ccaaatcttt cttcaggaat
     3121 atgtggctaa tctccttaag tcggccttcc ctcacctaca agatgctcaa gtaaagctct
     3181 ttgtgacagg gcttttcagc ttaaatcaag atattcctgc tttcaaggaa catttaagag
     3241 atttcctagt tcaaataaag gaatttgcag gtgaagacac ttctgatttg tttttggaag
     3301 agagagaaat agccctacgg caggctgatg aagagaaaca taaacgtcaa atgtctgtcc
     3361 ctggcatctt taatccacat gagattccag aagaaatgtg tgattgattg ccaactttct
     3421 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     3481 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
     3541 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     3601 acattgcaca agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg
     3661 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     3721 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     3781 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     3841 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     3901 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     3961 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     4021 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     4081 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     4141 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     4201 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     4261 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     4321 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     4381 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     4441 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     4501 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     4561 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     4621 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     4681 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     4741 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc
     4801 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     4861 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4921 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4981 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5041 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5101 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5161 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5221 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5281 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5341 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5401 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5461 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5521 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5581 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5641 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     5701 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     5761 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     5821 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     5881 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     5941 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     6001 ttgatgcct
//
