LOCUS       sequence-166761-        4575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing EYS ,part of the Target Accelerator 
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_EYS_p.N269H
FEATURES             Location/Qualifiers
     source          1..4575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1975..2074)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2091..2110)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2092..2110)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2115..2131)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2115..2131)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2562..3353
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3446..4034
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3935..3954
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4188..4205
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4364..4391)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4483..4569)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgactgac aaatcaatcg tcattctgag cctgatggtt tttcacagct
      241 ctttcataaa tggaaaaaca tgtagacggc aattggtgga agaatggcat ccacaaccct
      301 catcatatgt ggtaaattgg acactaacag aaaacatctg cttggacttc tacagagatt
      361 gctggttttt gggtgtaaac actaaaatag atacttcagg caatcaagct gttccccaga
      421 tttgcccttt gcaaattcaa ttaggagata tccttgtaat ttcttctgaa ccatctcttc
      481 aatttccaga aataaatttg atgaatgttt ctgaaacatc tttcgttggc tgtgtgcaaa
      541 ataccacaac ggaagatcag ttactttttg gctgcagact aaaaggaatg cacactgtta
      601 attctaagtg gctgagtgtt gggacacatt attttatcac agttatggca agtggtccat
      661 caccttgtcc actgggactt cgactaaatg tgacagtgaa acagcagttc tgccaggaat
      721 ctctgagttc agaattttgc tctggtcatg gtaaatgtct tagtgaagct tggagcaaga
      781 catatagctg ccattgccag cctccatttt ctggaaaata ctgccaggaa cttgatgcat
      841 gttcttttaa accatgtaaa aataatggca gttgcattaa taaaagagaa aattgggatg
      901 agcaagcata tgaatgtgtc tgtcacccac catttacagg aaagaattgc tcagaaataa
      961 ttggccagtg tcaaccacat gtctgtttcc atggacactg cagcaatatt acttcaaata
     1021 gtttcatttg tgaatgtgat gagcaatttt caggtccatt ctgtgaggtg tcagcaaaac
     1081 cttgtgtttc tctgcttttt tggaaaagag gaatttgccc aaatagcagt tctgcttata
     1141 cttatgaatg cccaaaagga tcttccagcc aaaatggtga aactgatgtc agtgagtttt
     1201 cattagtacc atgtcagaat ggtactgact gcatcaaaat atcaaatgat gttatgtgca
     1261 tctgttcacc aatatttaca gatttgcttt gtaagagcat tcaaacatca tgtgagtcat
     1321 ttcctttgag gaataatgct acatgtaaga aatgtgagaa agattatcct tgcagctgta
     1381 tatcaggatt tactgaaaaa aactgtgaga aagcaattga ccactgtaaa ctgctcagca
     1441 tcaactgtct gaatgaagaa tggtgtttca atataattgg aagattcaaa tatgtatgca
     1501 ttccagggtg cacaaaaaat ccatgttggt ttttgaagaa tgtttaccta attcatcaac
     1561 acctctgcta ctgtggagtc accttccatg gtatttgcca agataaaggt cctgctcaat
     1621 ttgaatatgt gtggcaattg ggatttgcag gatctgaagg cgaaaagtgc caaggggtta
     1681 ttgatgccta tttctttctg gctgcaaact gcactgaaga tgcaacctat gtgaacgatc
     1741 ctgaagataa taattcttca tgttggttcc cacatgaagg cacaaaagag atttgtgcaa
     1801 atggatgcag ttgtttgagt gaagaagaca gtcaggaata tcggtatcta tgttttctca
     1861 gatgggctgg caacatgtat ctggaaaata caactgatga tcaagaaaat gagtgtcaac
     1921 atgaagctgt ttgtaaagat gaaattaata gacccaggta tttatgtatc attttgccaa
     1981 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac
     2041 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg
     2101 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     2161 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca
     2221 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga
     2281 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     2341 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     2401 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     2461 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     2521 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg
     2581 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     2641 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     2701 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     2761 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     2821 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     2881 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     2941 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     3001 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     3061 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     3121 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     3181 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     3241 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     3301 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga
     3361 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     3421 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3481 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3541 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3601 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3661 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3721 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3781 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3841 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3901 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3961 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4021 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4081 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4141 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4201 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     4261 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     4321 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     4381 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     4441 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     4501 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     4561 ttttatttga tgcct
//
