LOCUS       sequence-166763-        4611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing NFE2L2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_NFE2L2_p.N160S
FEATURES             Location/Qualifiers
     source          1..4611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2011..2110)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2127..2146)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2128..2146)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2151..2167)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2151..2167)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2598..3389
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3482..4070
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3971..3990
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4224..4241
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4400..4427)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4519..4605)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgatggac ttggagctgc cgccgccggg actcccgtcc cagcaggaca
      241 tggatttgat tgacatactt tggaggcaag atatagatct tggagtaagt cgagaagtat
      301 ttgacttcag tcagcgacgg aaagagtatg agctggaaaa acagaaaaaa cttgaaaagg
      361 aaagacaaga acaactccaa aaggagcaag agaaagcctt tttcgctcag ttacaactag
      421 atgaagagac aggtgaattt ctcccaattc agccagccca gcacatccag tcagaaacca
      481 gtggatctgc caactactcc caggttgccc acattcccaa atcagatgct ttgtactttg
      541 atgactgcat gcagcttttg gcgcagacat tcccgtttgt agatgacaat gaggtttctt
      601 cggctacgtt tcagtcactt gttcctgata ttcccggtca catcgagagc ccagtcttca
      661 ttgctactag tcaggctcag tcacctgaaa cttctgttgc tcaggtagcc cctgttgatt
      721 tagacggtat gcaacaggac attgagcaag tttgggagga gctattatcc attcctgagt
      781 tacagtgtct taatattgaa aatgacaagc tggttgagac taccatggtt ccaagtccag
      841 aagccaaact gacagaagtt gacaattatc atttttactc atctataccc tcaatggaaa
      901 aagaagtagg taactgtagt ccacattttc ttaatgcttt tgaggattcc ttcagcagca
      961 tcctctccac agaagacccc aaccagttga cagtgaactc attaaattca gatgccacag
     1021 tcaacacaga ttttggtgat gaattttatt ctgctttcat agctgagccc agtatcagca
     1081 acagcatgcc ctcacctgct actttaagcc attcactctc tgaacttcta aatgggccca
     1141 ttgatgtttc tgatctatca ctttgcaaag ctttcaacca aaaccaccct gaaagcacag
     1201 cagaattcaa tgattctgac tccggcattt cactaaacac aagtcccagt gtggcatcac
     1261 cagaacactc agtggaatct tccagctatg gagacacact acttggcctc agtgattctg
     1321 aagtggaaga gctagatagt gcccctggaa gtgtcaaaca gaatggtcct aaaacaccag
     1381 tacattcttc tggggatatg gtacaaccct tgtcaccatc tcaggggcag agcactaacg
     1441 tgcatgatgc ccaatgtgag aacacaccag agaaagaatt gcctgtaagt cctggtcatc
     1501 ggaaaacccc attcacaaaa gacaaacatt caagccgctt ggaggctcat ctcacaagag
     1561 atgaacttag ggcaaaagct ctccatatcc cattccctgt agaaaaaatc attaacctcc
     1621 ctgttgttga cttcaacgaa atgatgtcca aagagcagtt caatgaagct caacttgcat
     1681 taattcggga tatacgtagg aggggtaaga ataaagtggc tgctcagaat tgcagaaaaa
     1741 gaaaactgga aaatatagta gaactagagc aagatttaga tcatttgaaa gatgaaaaag
     1801 aaaaattgct caaagaaaaa ggagaaaatg acaaaagcct tcacctactg aaaaaacaac
     1861 tcagcacctt atatctcgaa gttttcagca tgctacgtga tgaagatgga aaaccttatt
     1921 ctcctagtga atactccctg cagcaaacaa gagatggcaa tgttttcctt gttcccaaaa
     1981 gtaagaagcc agatgttaag aaaaactgat tgccaacttt cttgtacaaa gttggcatta
     2041 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     2101 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     2161 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     2221 atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     2281 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     2341 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca
     2401 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg
     2461 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
     2521 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
     2581 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag
     2641 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     2701 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     2761 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     2821 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     2881 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     2941 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     3001 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     3061 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     3121 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     3181 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     3241 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     3301 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
     3361 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta
     3421 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3481 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3541 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3601 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3661 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3721 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3781 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3841 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     3901 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3961 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4021 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4081 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4141 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4201 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     4261 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     4321 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     4381 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     4441 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
     4501 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     4561 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc t
//
