LOCUS       sequence-166766-        4698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing POT1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_POT1_p.L265H
FEATURES             Location/Qualifiers
     source          1..4698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2098..2197)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2214..2233)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2215..2233)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2238..2254)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2238..2254)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2685..3476
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3569..4157
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4058..4077
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4311..4328
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4487..4514)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4606..4692)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgtctttg gttccagcaa caaattatat atatacaccc ctgaatcaac
      241 ttaagggtgg tacaattgtc aatgtctatg gtgttgtgaa gttctttaag cccccatatc
      301 taagcaaagg aactgattat tgctcagttg taactattgt ggaccagaca aatgtaaaac
      361 taacttgcct gctctttagt ggaaactatg aagcccttcc aataatttat aaaaatggag
      421 atattgttcg ctttcacagg ctgaagattc aagtatataa aaaggagact cagggtatca
      481 ccagctctgg ctttgcatct ttgacgtttg agggaacttt gggagcccct atcatacctc
      541 gcacttcaag caagtatttt aacttcacta ctgaggacca caaaatggta gaagccttac
      601 gtgtttgggc atctactcat atgtcaccgt cttggacatt actaaaattg tgtgatgttc
      661 agccaatgca gtattttgac ctgacttgtc agctcttggg caaagcagaa gtggacggag
      721 catcatttct tctaaaggta tgggatggca ccaggacacc atttccatct tggagagtct
      781 taatacaaga ccttgttctt gaaggtgatt taagtcacat ccatcggcta caaaatctga
      841 caatagacat tttagtctac gataaccatg ttcatgtggc aagatctctg aaggttggaa
      901 gctttcttag aatctatagc cttcatacca aacttcaatc aatgaattca gagaatcaga
      961 caatgttaag tttagagttt catcatcatg gaggtaccag ttacggtcgg ggaatcaggg
     1021 tcttgccaga aagtaactct gatgtggatc aactgaaaaa ggatttagaa tctgcaaatt
     1081 tgacagccaa tcagcattca gatgttatct gtcaatcaga acctgacgac agctttccaa
     1141 gctctggatc agtatcatta tacgaggtag aaagatgtca acagctatct gctacaatac
     1201 ttacagatca tcagtatttg gagaggacac cactatgtgc cattttgaaa caaaaagctc
     1261 ctcaacaata ccgcatccga gcaaaattga ggtcatataa gcccagaaga ctatttcagt
     1321 ctgttaaact tcattgccct aaatgtcatt tgctgcaaga agttccacat gagggcgatt
     1381 tggatataat ttttcaggat ggtgcaacta aaaccccaga tgtcaagcta caaaatacat
     1441 cattatatga ttcaaaaatc tggaccacta aaaatcaaaa aggacgaaaa gtagcagttc
     1501 attttgtgaa aaataatggt attctcccgc tttcaaatga atgtctactt ttgatagaag
     1561 gaggtacact cagtgaaatt tgcaaactct cgaacaagtt taatagtgta attcctgtga
     1621 gatctggcca cgaagacttg gaacttttgg acctttcagc accatttctt atacaaggaa
     1681 caatacatca ctatggatgt aaacagtgtt ctagtttgag atccatacaa aatctaaatt
     1741 ccctggttga taaaacatcg tggattcctt cttctgtggc agaagcactg ggtattgtac
     1801 ccctccaata tgtgtttgtt atgaccttta cacttgatga tggaacagga gtactagaag
     1861 cctatctcat ggattctgac aaattcttcc agattccagc atcagaagtt ctgatggatg
     1921 atgaccttca gaaaagtgtg gatatgatca tggatatgtt ttgtcctcca ggaataaaaa
     1981 ttgatgcata tccgtggttg gaatgcttca tcaagtcata caatgtcaca aatggaacag
     2041 ataatcaaat ttgctatcag atttttgaca ccacagttgc agaagatgta atctgattgc
     2101 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     2161 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     2221 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     2281 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     2341 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     2401 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     2461 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     2521 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     2581 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     2641 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     2701 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     2761 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     2821 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     2881 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     2941 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     3001 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     3061 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     3121 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     3181 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     3241 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     3301 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     3361 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     3421 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     3481 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     3541 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3601 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3661 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3721 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3781 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3841 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3901 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3961 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4021 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4081 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4141 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4201 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4261 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4321 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4381 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4441 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     4501 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     4561 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     4621 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     4681 tcgttttatt tgatgcct
//
