LOCUS       sequence-166767-         597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  induce expression of dipeptide repeat PR in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Jovicic A, Mertens J, Boeynaems S, Bogaert E, Chai N, Yamada SB, 
            Paul JW 3rd, Sun S, Herdy JR, Bieri G, Kramer NJ, Gage FH, Van Den 
            Bosch L, Robberecht W, Gitler AD
  TITLE     Modifiers of C9orf72 dipeptide repeat toxicity connect 
            nucleocytoplasmic transport defects to FTD/ALS.
  JOURNAL   Nat Neurosci. 2015 Sep;18(9):1226-9. doi: 10.1038/nn.4085.
  PUBMED    26308983
REFERENCE   2  (bases 1 to 597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn"; 
            journalName: "Nat Neurosci"; date: "2015-09"; volume: "18"; issue: 
            "9"; pages: "1226-9"
COMMENT     Sequence Label: pAG303-Gal-PR50
FEATURES             Location/Qualifiers
     source          1..597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(8..255)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     221..239
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     257..273
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     258..274
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    307..331
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(342..365)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(384..413)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cgaggccgca aattaaagcc ttcgagcgtc ccaaaacctt ctcaagcaag gttttcagta
       61 taatgttaca tgcgtacacg cgtctgtaca gaaaaaaaag aaaaatttga aatataaata
      121 acgttcttaa tactaacata actataaaaa aataaatagg gacctagact tcaggttgtc
      181 taactccttc cttttcggtt agagcggatg tggggggagg gcgtgaatgt aagcgtgaca
      241 taactaatta catgactcga ggtcgacggt atcgataagc ttgatatcga attcctgcag
      301 cccatcacca ctttgtacaa gaaagctggg ttagatcttt acttatcgtc gtcatccttg
      361 taatccagga tatcatttgc tgccagatcc tcttctgaga tgagtttctg ctcacgtggt
      421 cgaggacgcg gtcgaggtcg tggtcgagga cgaggtcgag gacgcggtcg aggtcgtggt
      481 cgaggacgag gccgaggacg agggcgtggc cgtggccgtg gacgaggccg aggacgagga
      541 cgtggtcgag gacgcggtcg aggtcgtggt cgaggacgag gtcgaggacg cggtcga
//
