LOCUS       sequence-166770-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  target  miRNA824  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM824
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     114..133
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(275..280,281..297,298..303)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(406..425)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atctcagaat tcatggatcc taggtgagtc tagagagtta attaagaaag gcggacaggt
       61 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca ggggggaaac
      121 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg ccgatttttg
      181 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcgga tcacaacaaa
      241 aagcccgctc attaggcggg ctaaattctc atgtttgaca gcttatcatc gataagcttt
      301 aatgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaga aatcactgga
      361 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
      421 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
      481 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
      541 aatgctcatc cggagttccg tatggcaatg aaagacggtg agctggtaat atgggatagt
      601 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
      661 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
      721 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
      781 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatg
//
