LOCUS       sequence-16678-e        1383 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3502
FEATURES             Location/Qualifiers
     source          1..1383
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(129..145)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(130..146)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(243..271)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctaaagggac aaaagctgga gctccaccgc ggtggcggcc gctctagaac tagnggatcc
       61 accggttcca tggctagcca cgtgacgcgt ggatcccccg ggctgcagga attcgatatc
      121 aagcttatcg ataccgtcga cctcgagggg gggcccggta cgatatacta actaacaaga
      181 attatctaat ttttcaggta ccggtagaaa aaatgagtaa aggagaagaa cttttcactg
      241 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
      301 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
      361 ctggaaaact acctgttcca tgggtaagtt taaacatata tatactaact aaccctgatt
      421 atttaaattt tcagccaaca cttgtcacta ctctcactta tggtgttcaa tgcttctcga
      481 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg
      541 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt aagtttaaac
      601 agttcggtac taactaacca tacatattta aattttcagg tgctgaagtc aagtttgaag
      661 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
      721 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
      781 aacaaaagaa tggaatcaaa gttgtaagtt taaacatgat tttactaact aactaatctg
      841 atttaaattt tcagaacttc aaaattagac acaacattga agatggaagc gttcaactag
      901 cagaccatta tcaacaaaat actcccaatt ggcgatggcc ctgtcctttt accagacaac
      961 cattacctgt ccccacaatc tgcccctttc gaangatccn acgaaaaaaa aagaccacnt
     1021 gggtccntct tgagtttgna acngctgctg ggattcccnt ggcagggnga actntcnaag
     1081 naagttttac ttttatcnna cgaangaaaa agcncccccc ngggggggcc cccnaaatna
     1141 ggganccccg gttcctggct agcccgggac cgggnatccc cggncgcnga atccaatnaa
     1201 nntacnancc ncccacccng ggggggccgg anccattccc cnnaanggnc naatcccnnc
     1261 ccngccnctt ttaaangcgg gnggnaaccg ggttccaana nnntggacaa ccctttcccg
     1321 gggaaaanaa gccccccncc ccccntcccn gagggagnac ccnnncctnc ccgggggnnc
     1381 ccc
//
