LOCUS       sequence-166788-        4500 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TRAF3 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TRAF3_p.D463N
FEATURES             Location/Qualifiers
     source          1..4500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2379..2478)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2495..2514)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2496..2514)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2519..2535)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2519..2535)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2966..3757
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3850..4438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4339..4358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggagtcga gtagaaagat ggactctcct ggcgcgctgc agactaaccc
      721 gccgctaaag ctgcacactg accgcagtgc tgggacgcca gtttttgtcc ctgaacaagg
      781 aggttacaag gaaaagtttg tgaagaccgt ggaggacaag tacaagtgtg agaagtgcca
      841 cctggtgctg tgcagcccga agcagaccga gtgtgggcac cgcttctgcg agagctgcat
      901 ggcggccctg ctgagctctt caagtccaaa atgtacagcg tgtcaagaga gcatcgttaa
      961 agataaggtg tttaaggata attgctgcaa gagagaaatt ctggctcttc agatctattg
     1021 tcggaatgaa agcagaggtt gtgcagagca gttaatgctg ggacatctgc tggtgcattt
     1081 aaaaaatgat tgccattttg aagaacttcc atgtgtgcgt cctgactgca aagaaaaggt
     1141 cttgaggaaa gacctgcgag accacgtgga gaaggcgtgt aaataccggg aagccacatg
     1201 cagccactgc aagagtcagg ttccgatgat cgcgctgcag aaacacgaag acaccgactg
     1261 tccctgcgtg gtggtgtcct gccctcacaa gtgcagcgtc cagactctcc tgaggagcga
     1321 gttgagtgca cacttgtcag agtgtgtcaa tgcccccagc acctgtagtt ttaagcgcta
     1381 tggctgcgtt tttcagggga caaaccagca gatcaaggcc cacgaggcca gctccgccgt
     1441 gcagcacgtc aacctgctga aggagtggag caactcgctc gaaaagaagg tttccttgtt
     1501 gcagaatgaa agtgtagaaa aaaacaagag catacaaagt ttgcacaatc agatatgtag
     1561 ctttgaaatt gaaattgaga gacaaaagga aatgcttcga aataatgaat ccaaaatcct
     1621 tcatttacag cgagtgatag acagccaagc agagaaactg aaggagcttg acaaggagat
     1681 ccggcccttc cggcagaact gggaggaagc agacagcatg aagagcagcg tggagtccct
     1741 ccagaaccgc gtgaccgagc tggagagcgt ggacaagagc gcggggcaag tggctcggaa
     1801 cacaggcctg ctggagtccc agctgagccg gcatgaccag atgctgagtg tgcacgacat
     1861 ccgcctagcc gacatggacc tgcgcttcca ggtcctggag accgccagct acaatggagt
     1921 gctcatctgg aagattcgcg actacaagcg gcggaagcag gaggccgtca tggggaagac
     1981 cctgtccctt tacagccagc ctttctacac tggttacttt ggctataaga tgtgtgccag
     2041 ggtctacctg aacgggaacg ggatggggaa ggggacgcac ttgtcgctgt tttttgtcat
     2101 catgcgtgga gaatatgatg ccctgcttcc ttggccgttt aagcagaaag tgacactcat
     2161 gctgatggat caggggtcct ctcgacgtca tttgggagat gcattcaagc ccgaccccaa
     2221 cagcagcagc ttcaagaagc ccactggaga gatgaatatc gcctctggct gcccagtctt
     2281 tgtggcccaa actgttctag aaaatgggac atatattaaa gatgatacaa tttttattaa
     2341 agtcatagtg gatacttcgg atctgcccga tccctgattg ccaactttct tgtacaaagt
     2401 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     2461 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     2521 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     2581 agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg cctcgacttc
     2641 gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca
     2701 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa
     2761 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     2821 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg
     2881 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag
     2941 tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag tatcgactca
     3001 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
     3061 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
     3121 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
     3181 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
     3241 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
     3301 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
     3361 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
     3421 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
     3481 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg
     3541 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
     3601 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
     3661 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
     3721 cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc catgaccaaa
     3781 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3841 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3901 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3961 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4021 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4081 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4141 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4201 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4261 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4321 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4381 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4441 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
