LOCUS       sequence-166789-        4281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SLC2A3 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SLC2A3_p.K49N
FEATURES             Location/Qualifiers
     source          1..4281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2160..2259)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2276..2295)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2277..2295)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2300..2316)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2300..2316)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2747..3538
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3631..4219
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4120..4139
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atggggacac agaaggtcac cccagctctg atatttgcca tcacagttgc
      721 tacaatcggc tctttccaat ttggctacaa cactggggtc atcaatgctc ctgagaagat
      781 cataaaggaa tttatcaata aaactttgac ggacaatgga aatgccccac cctctgaggt
      841 gctgctcacg tctctctggt ccttgtctgt ggccatattt tccgtcgggg gtatgatcgg
      901 ctccttttcc gtcggactct tcgtcaaccg ctttggcagg cgcaattcaa tgctgattgt
      961 caacctgttg gctgtcactg gtggctgctt tatgggactg tgtaaagtag ctaagtcggt
     1021 tgaaatgctg atcctgggtc gcttggttat tggcctcttc tgcggactct gcacaggttt
     1081 tgtgcccatg tacattggag agatctcgcc tactgccctg cggggtgcct ttggcactct
     1141 caaccagctg ggcatcgttg ttggaattct ggtggcccag atctttggtc tggaattcat
     1201 ccttgggtct gaagagctat ggccgctgct actgggtttt accatccttc ctgctatcct
     1261 acaaagtgca gcccttccat tttgccctga aagtcccaga tttttgctca ttaacagaaa
     1321 agaagaggag aatgctaagc agatcctcca gcggttgtgg ggcacccagg atgtatccca
     1381 agacatccag gagatgaaag atgagagtgc aaggatgtca caagaaaagc aagtcaccgt
     1441 gctagagctc tttagagtgt ccagctaccg acagcccatc atcatttcca ttgtgctcca
     1501 gctctctcag cagctctctg ggatcaatgc tgtgttctat tactcaacag gaatcttcaa
     1561 ggatgcaggt gttcaagagc ccatctatgc caccatcggc gcgggtgtgg ttaatactat
     1621 cttcactgta gtttctctat ttctggtgga aagggcagga agaaggactc tgcatatgat
     1681 aggccttgga gggatggctt tttgttccac gctcatgact gtttctttgt tattaaagga
     1741 taactataat gggatgagct ttgtctgtat tggggctatc ttggtctttg tagccttctt
     1801 tgaaattgga ccaggcccca ttccctggtt tattgtggcc gaactcttca gccagggccc
     1861 ccgcccagct gcgatggcag tggccggctg ctccaactgg acctccaact tcctagtcgg
     1921 attgctcttc ccctccgctg ctcactattt aggagcctac gtttttatta tcttcaccgg
     1981 cttcctcatt accttcttgg cttttacctt cttcaaagtc cctgagaccc gtggcaggac
     2041 ttttgaggat atcacacggg cctttgaagg gcaggcacac ggtgcagata gatctggaaa
     2101 ggacggcgtc atggagatga acagcatcga gcctgctaag gagaccacca ccaatgtcta
     2161 cccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     2221 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     2281 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     2341 tctctgatgt tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca
     2401 ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa
     2461 acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     2521 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     2581 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     2641 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     2701 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt
     2761 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     2821 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     2881 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     2941 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     3001 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     3061 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     3121 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     3181 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     3241 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     3301 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     3361 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     3421 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     3481 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc
     3541 ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     3601 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3661 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3721 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3781 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3841 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3901 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3961 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4021 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4081 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4141 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4201 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4261 ctggcctttt gctcacatgt t
//
