LOCUS       sequence-166793-       10575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  P. falciparum integration plasmid for N-terminal gene fusions and 
            placing loxP sites using SLI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Birnbaum J, Flemming S, Reichard N, Soares AB, Mesen-Ramirez P, 
            Jonscher E, Bergmann B, Spielmann T
  TITLE     A genetic system to study Plasmodium falciparum protein function.
  JOURNAL   Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223.
  PUBMED    28288121
REFERENCE   2  (bases 1 to 10575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223."
COMMENT     Sequence Label: pSLI-N-GFP-2xFKBP-loxP(K13)
FEATURES             Location/Qualifiers
     source          1..10575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2472..2489)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2472..2488)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2481..2503)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2697..2716)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2816..2838
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2876..2894)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2962..3066
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3067..3135,3136..3927)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3285..3304)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4098..4686
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4587..4606
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4840..4857
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4974..4995
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5010..5015,5016..5033,5034..5040)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5048..5064
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5053..5075
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5072..5088
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5072..5088
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5106..5124
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5106..5123
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             5695..5724
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5728..5757
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(5758..5791)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5797..5850
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6796..6849
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(6886..6914)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     7509..7529
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             7642..7962
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             7969..8289
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     protein_bind    complement(10531..10564)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggatatggca gcttaatgtt cgtttttctt atttatatat ttataccaat tgattgtatt
       61 tataactgta aaaatgtgta tgttgtgtgc atattttttt ttgtgcatgc acatgcatgt
      121 aaatagctaa aattatgaac attttatttt ttgttcagaa aaaaaaaact ttacacacat
      181 aaaatggcta gtatgaatag ccatatttta tataaattaa atcctatgaa tttatgacca
      241 tattaaaaat ttagatattt atggaacata atatgtttga aacaataaga caaaattatt
      301 attattatta ttatttttac tgttataatt atgtgtctcc ttcaatgatt cataaatagt
      361 tggacttgat ttttaaaatg tttataatat gattagcata gttaaataaa aaaagttgaa
      421 aaattaaaaa aaaacatata aacacaaatg atggtttttc cttcaatttc gatatcaatt
      481 tatagaaaca aaatatatac ttgtataatt ttattttttt atataaatca ttacatatat
      541 aattatacaa tattttttct aagagataat tatatattaa tatatataaa aaaaggtgtt
      601 tttttttttt ttttttattt ttatttttat tttatggtaa tattttattt tccttatttt
      661 ataaattata ttagtttata tgtgattaat tttatatatt atcaatttat atatttttaa
      721 atgcttactt aattatcttt tttttttttt tttttttttt ttcccctctt tttatattaa
      781 tttatttttg aaaaaattga tatatatata tatatataat atatatatat acatgtagta
      841 gtattaaaca atgtataata tatataaata atatatttat atatttcatt tcaattttaa
      901 ttttttttgg tttttttttt ttttcttttt gtcatattta aaaaaaatta tattcatata
      961 agttatgcat tttttataaa cattattcaa tatatgtata atataatata tatatatata
     1021 ttaatgtatt attccaatgt gcatgataaa agaaaaaaat aatatttata aaaaaaaaga
     1081 aaaataaaac aaaaaaagaa aaaaaaaaaa aaaaaaaaaa aaatacaaaa ataaataata
     1141 taatttataa ttatatattc ttgtcacaat aaaaatatat atatatatat atatatttat
     1201 aatatgtata ttttaaacta gaaaaggaat aactaatatt ttatttatta tcattcaaga
     1261 tttatatttt ataataataa atacctaata gaaatatatc aggatccatg catggttcgc
     1321 taaactgcat cgtcgctgtg tcccagaaca tgggcatcgg caagaacggg gactacccct
     1381 ggccaccgct caggaacgaa tttagatatt tccagagaat gaccacaacc tcttcagtag
     1441 aaggtaaaca gaatctggtg attatgggta agaagacctg gttctccatt cctgagaaga
     1501 atcgaccttt aaagggtaga attaatttag ttctcagcag agaactcaag gaacctccac
     1561 aaggagctca ttttctttcc agaagtctag atgatgcctt aaaacttact gaacaaccag
     1621 aattagcaaa taaagtagac atggtctgga tagttggtgg cagttctgtt tataaggaag
     1681 ccatgaatca cccaggccat cttaaactat ttgtgacaag gatcatgcaa gactttgaaa
     1741 gtgacacgtt ttttccagaa attgatttgg agaaatataa acttctgcca gaatacccag
     1801 gtgttctctc tgatgtccag gaggagaaag gcattaagta caaatttgaa gtatatgaga
     1861 agaatgatta agcttattta ataatagatt aaaaatatta taaaaataaa aacataaaca
     1921 cagaaattac aaaaaaaata catatgaatt ttttttttgt aatcttcctt ataaatatag
     1981 aataatgaat catataaaac atatcattat tcatttattt acatttaaaa ttattgtttc
     2041 agtatcttta atttattatg tatatataaa aataacttac aattttatta ataaacaata
     2101 tatgtttatt aattcatgtt ttgtaattta tgggatagcg atttttttta ctgtctgtat
     2161 ttttcttttt taattatgtt ttaattgtat tttattttta ttattgttct ttttatagta
     2221 ttattttaaa acaaaatgta ttttctaaga acttataata ataataaata taaattttaa
     2281 taaaaattat atttatcttt tacaatatga acataaagta caacattaat atatagcttt
     2341 taatattttt attcctaatc atgtaaatct taaatttttc tttttaaaca tatgttaaat
     2401 atttatttct cattatatat aagaacatat ttattaaatc tagaattcta tagtgagtcg
     2461 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     2521 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     2581 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     2641 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     2701 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     2761 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     2821 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     2881 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     2941 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3001 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3061 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3121 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3181 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3241 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3301 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3361 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3421 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3481 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3541 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3601 acgatgcctg tagcaatgcc aacaacgttg cgcaaactat taactggcga actacttact
     3661 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     3721 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     3781 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     3841 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3901 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     3961 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4021 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4081 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4141 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4201 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     4261 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4321 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4381 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4441 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4501 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4561 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4621 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4681 tcgaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4741 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     4801 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     4861 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     4921 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     4981 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     5041 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     5101 aagctattta ggtgacacta tagaatactc gcggccgcta aagatgcagc aaatcttata
     5161 aatgatgatt ctggattaaa caatttaaaa aaattttcaa ctgtaaataa tgtaaatgat
     5221 acttatgaaa agaaaattat tgaaacggaa ttaagtgatg ctagtgattt tgaaaatatg
     5281 gtaggtgatt taagaattac atttattaat tggttaaaaa agacacaaat gaattttatt
     5341 cgagaaaaag ataaattatt taaagataag aaagaactag aaatggaaag agtacgattg
     5401 tacaaagaat tagaaaaccg taaaaatatt gaagaacaga aattacatga tgaaagaaag
     5461 aaattagata ttgatatatc taatggttat aaacaaataa aaaaagaaaa agaagaacat
     5521 aggaaacgat ttgatgaaga aagattaaga tttttacaag aaatcgataa aattaaatta
     5581 gtattatatt tagaaaaaga aaaatattat caagaatata aaaattttga gaatgataaa
     5641 aaaaaaattg ttgatgcaaa tattgctact gaaactatga ttgatggttt aaacgagcag
     5701 aagttaatat cagaagagga tttgggtgaa caaaaactca taagcgaaga agatttaata
     5761 acttcgtata gcatacatta tacgaagtta tccggagaag gaagaggaag tttattaaca
     5821 tgtggagatg tagaagaaaa tccaggacca atgacagcca gtttaactac caagttcttg
     5881 aacaatacct atgaaaaccc atttatgaat gcatccggtg ttcattgcat gactacacaa
     5941 gaattagatg aattagcaaa ctctaaagct ggcgcattca ttacaaagag tgctacaacc
     6001 ttagaaagag aaggtaaccc tgaaccacgt tacatttctg tccctctagg cagtatcaac
     6061 tccatgggtt taccaaacga aggtatcgac tactatttgt cctatgtatt aaaccgtcaa
     6121 aagaattatc ctgatgcacc tgctattttc ttctcagttg ctggtatgag cattgatgaa
     6181 aatttaaatt tgttgaggaa aatccaagat agcgaattca acggtattac cgagttaaac
     6241 ttgtcttgtc ctaatgtgcc tgggaaacca caagttgctt atgactttga cttgacaaag
     6301 gaaaccttgg aaaaggtttt tgcctttttc aaaaaacctc ttggtgtcaa gttgcctcct
     6361 tattttgatt ttgcccattt tgatatcatg gcaaaaatat tgaacgagtt cccattagct
     6421 tatgtcaact ctatcaatag tataggaaat ggtcttttca ttgatgtgga gaaggagagt
     6481 gtagtagtga agccaaagaa tggtttcggg ggtattggag gtgaatatgt taagccaacc
     6541 gcgctcgcca atgttcgtgc attttacact cgtttgagac ctgaaatcaa agttatcggt
     6601 acaggtggaa ttaagtccgg taaggatgca tttgaacatc ttctatgtgg tgcctctatg
     6661 ctacagattg gtacagaatt acaaaaagag ggcgtcaaga tttttgaacg tatcgaaaaa
     6721 gaattaaaag acataatgga agctaagggt tatacatcca tagatcagtt ccgtgggaag
     6781 ttgaacagca ttggtgaagg tagaggttct ttgttgactt gtggtgatgt tgaagaaaat
     6841 ccaggtccag ctagcatgag taaaggagaa gaacttttca ctggagttgt cccaattctt
     6901 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
     6961 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actaccagtt
     7021 ccatggccaa cacttgtcac tactttcgcg tatggtcttc aatgctttgc gagataccca
     7081 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
     7141 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
     7201 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     7261 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
     7321 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
     7381 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
     7441 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
     7501 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgag
     7561 ctctacaaac ctagctcagg attgagatca agatctgctg ctgctggtgc tggtggtgct
     7621 gctagagctg ctctgcagag aggagtacaa gttgaaacaa tatcaccagg agatggtcgt
     7681 acatttccaa aaagaggtca aacttgtgtt gtacattata ctggaatgct tgaagatgga
     7741 aagaaatttg attcatctcg tgatagaaat aaaccattta aatttatgct aggtaaacaa
     7801 gaagtaatac gaggttggga agaaggagtt gctcaaatga gtgtaggtca aagagcaaaa
     7861 cttactatat ctccagatta tgcttatggt gcaactggac atccaggtat aattccacct
     7921 catgcaactc ttgtatttga tgtggagctt ctaaaactag aaactagagg tgttcaggtt
     7981 gaaacaattt cacctggaga tggcagaacc tttcctaaaa gaggacagac ttgcgtagtt
     8041 cattatacag gcatgctaga ggatggtaag aaatttgatt ctagtcgaga tagaaataag
     8101 ccattcaagt ttatgctagg taaacaggaa gtaataagag gttgggaaga gggtgtagca
     8161 cagatgtcag ttggacaaag agcaaagtta acaatatcac cagattatgc atacggtgca
     8221 acaggccatc ctggcatcat ccctccacat gcaactttag tattcgacgt tgaattgtta
     8281 aagttagaga caacgcgtgc tagaggtgct gctgctggtg ctggaggtgc aggtagacct
     8341 aggatggagg gtgagaaggt taagactaaa gctaacagta taagtaactt cagtatgact
     8401 tacgacagag agagtggagg gaatagtaac tcagacgaca agagtggttc aagttcagaa
     8461 aacgactcaa acagttttat gaacttaaca tctgacaaga acgaaaagac agagaacaac
     8521 tcatttttac ttaacaactc atcttacggt aacgtaaagg actctttatt ggagtctata
     8581 gacatgtcag tgttggacag taatttcgac tcaaagaagg acttcttgcc ttcaaacttg
     8641 tctaggactt tcaacaacat gagtaaggac aacattggta acaagtactt gaacaagtta
     8701 ttgaacaaga agaaggacac aataactaac gagaacaaca acataaacca caacaacaac
     8761 aacaacaact taactgctaa caacattaca aacaacttaa taaacaacaa catgaactca
     8821 ccttctataa tgaacacaaa taagaaggaa aacttcttgg acgctgctaa cttgattaac
     8881 gacgactcag gtttgaataa cttgaagaag ttctctacag ttaacaacgt taacgacaca
     8941 tacgagaaaa agataataga gaccgagttg tcagacgcat cagacttcga gaacatggtt
     9001 ggagacttga ggataacttt cataaactgg ttgaagaaaa ctcagatgaa cttcataaga
     9061 gagaaggaca agttgttcaa ggacaaaaag gagcttgaga tggagagggt tcgtttatat
     9121 aaggagttgg agaatcgtaa gaacatagag gagcaaaagt tgcacgacga gaggaaaaag
     9181 ttggacatag acatttcaaa cggatacaag cagattaaga aggagaagga ggagcacaga
     9241 aagcgtttcg acgaggagag gttgaggttc ttgcaggaga tagacaagat aaagttggtt
     9301 ttgtacttgg agaaggagaa gtactaccag gagtacaaga acttcgaaaa cgacaagaag
     9361 aagatagtag acgctaacat agcaacagag acaatgatag acataaacgt aggaggtgca
     9421 atattcgaga cttcaaggca cactttgact cagcagaagg actctttcat tgaaaagttg
     9481 ttgtcaggta ggcaccacgt tacaagggac aagcagggta ggattttttt ggacagagac
     9541 tcagaattgt tcaggataat tttaaatttc ttgaggaacc ccttgacaat tcctattcct
     9601 aaggacttgt ctgagtcaga ggctttatta aaggaggctg agttctacgg aataaagttc
     9661 ttgccttttc ctttggtttt ctgcattgga ggtttcgacg gagttgagta cttgaacagt
     9721 atggagttgt tggacatatc acagcagtgt tggcgaatgt gcactccaat gtcaactaag
     9781 aaggcatact tcggttctgc agttttaaac aactttttgt atgtattcgg aggaaacaat
     9841 tacgactaca aagcattgtt cgagacagag gtatacgaca ggttgaggga cgtttggtac
     9901 gtaagttcaa acttgaacat tccaaggagg aacaactgcg gagtaacctc taacggaagg
     9961 atatactgca taggaggtta cgacggatca tcaataattc ccaacgttga ggcttacgac
    10021 cacagaatga aggcttgggt tgaagtagct ccattaaaca ctccaaggtc tagtgcaatg
    10081 tgcgtagcat tcgacaacaa gatatacgtg ataggaggta caaacggaga aaggttgaac
    10141 tcaatagagg tttacgagga gaagatgaac aagtgggagc agttccctta cgcattgttg
    10201 gaggcaaggt caagtggtgc tgcattcaac tatttaaacc agatttacgt agtaggtgga
    10261 atagacaacg agcacaatat tttggactct gtagagcagt accagccttt caacaagagg
    10321 tggcagttcc ttaacggagt tcctgaaaag aagatgaact tcggtgcagc tactttaagt
    10381 gactcataca ttataactgg tggtgagaac ggggaggtac ttaactcttg ccactttttc
    10441 tcacctgaca ctaacgagtg gcaattagga ccttcattgt tggtaccaag gttcggacat
    10501 tcagtattga ttgctaacat ttgaaggcct ataacttcgt atagcataca ttatacgaag
    10561 ttattatgac tcgag
//
