LOCUS       sequence-166795-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of YFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Elden AC, Kim HJ, Hart MP, Chen-Plotkin AS, Johnson BS, Fang X, 
            Armakola M, Geser F, Greene R, Lu MM, Padmanabhan A, Clay-Falcone D,
            McCluskey L, Elman L, Juhr D, Gruber PJ, Rub U, Auburger G, 
            Trojanowski JQ, Lee VM, Van Deerlin VM, Bonini NM, Gitler AD
  TITLE     Ataxin-2 intermediate-length polyglutamine expansions are associated
            with increased risk for ALS.
  JOURNAL   Nature. 2010 Aug 26;466(7310):1069-75. doi: 10.1038/nature09320.
  PUBMED    20740007
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-08-26- 26"; volume: "466"; issue: "7310"; 
            pages: "1069-75"
COMMENT     Sequence Label: pcDNA3.2 YFP
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(40..64)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(93..112)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(94..112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(157..360)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(186..206)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        361..740
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     912..931
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gctcctcgcc cttgctcacc attttgttat ctccttcgaa gcctgctttt ttgtacaaac
       61 ttgttgatag cttaactagc cagcttgggt ctccctatag tgagtcgtat taatttcgat
      121 aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat agacctccca
      181 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
      241 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
      301 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
      361 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
      421 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
      481 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
      541 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
      601 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
      661 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
      721 actagtcaat aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa
      781 acgcctaacc ctaagcagat tcttcatgca attgtcggtc aagccttgcc ttgttgtagc
      841 ttaaattttg ctcgcgcact actcagcgac ctccaacaca caagcaggga gcagatactg
      901 gcttaactat gcggcatcag agcagattgt actgagagtg caccataggg gat
//
