LOCUS       sequence-166798-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sfCherry2(11) tagged heterochromatin protein1 in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
  TITLE     Improved split fluorescent proteins for endogenous protein labeling.
  JOURNAL   Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
  PUBMED    28851864
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: " 
            10.1038/s41467-017-00494-8"
COMMENT     Sequence Label: pEGFP_sfCherry2(11)_HP1
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(663..762)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(692..712)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttcttgtcgt cttttttgtc atcatcctct gaggggtagg aatgccacgt tagcctttcc
       61 tcatagaagg atatgacaac ctgtgggcac ttgacattgg cttccttggc agggaccagg
      121 tcagcctcat cagagttttt ccatttcatc aggaacatga gctctccact ggagtcagta
      181 gctccaataa tccgctctgg ctccaaaccc cgggcaaagc ctcgtggctt ttctgactct
      241 tctttcttct tctttggttt gctttcctct cctttatctt cagaatcaga atcagctttg
      301 cgcttgcctc cctctgactt atctgtctca tgagctgttt tctgtgactg tagaaactca
      361 gcaataaggt cagggcaatc cagattctct tctggctccc aagtgttgtc ctcatctgag
      421 aaacccttcc actttagaag atattccacc ttgcccttga caactcgccg atcaagaact
      481 ttttccacca catattcctc ttcctcttct tctagtacct cctccacttt cttcttgttt
      541 tgctttttcc ccatagatct gagccggacg gtgctgtgtc tggcctcggc tctctcgtac
      601 tgctccacga tggtgtacat tggcgaccgg tagcgctagc ggatctgacg gttcactaaa
      661 ccagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
      721 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
      781 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
      841 ttgatgtact gccaa
//
