LOCUS       sequence-16680-e        1343 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3505
FEATURES             Location/Qualifiers
     source          1..1343
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    23..130
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     47..63
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     47..63
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(97..113)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(98..114)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(171..199)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cctcctaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gaactagtgg
       61 atcccccggg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagggggg
      121 gcccggtacc ggtagaaaaa atgagtaaag gagaagaact tttcactgga gttgtcccaa
      181 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      241 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      301 ctgttccatg ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc
      361 agccaacact tgtcactact ctcacttatg gtgttcaatg cttctcgaga tacccagatc
      421 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
      481 ctatattttt caaagatgac gggaactaca agacacgtaa gtttaaacag ttcggtacta
      541 actaaccata catatttaaa ttttcaggtg ctgaagtcaa gtttgaaggt gatacccttg
      601 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      661 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
      721 gaatcaaagt tgtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc
      781 agaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
      841 aacaaaatac tccaattggc gatggnccct gtccttttac cagacaacca ttacctgtcc
      901 acacaatctg cccttttcga agatcccaac gaaaagagag acccacatgg tcctttcttg
      961 agtttggtaa cagcctgctg ggnattacac ntgggcatgg gatgaactat accaaatgcc
     1021 ccgggggnat cgggggganc tcccccgcgg gngggnggnc cnctcntaaa actagnggna
     1081 tcccccnggg ctgcnggnaa ttccaaatca agcttatcng ataccgccca acccnagggg
     1141 gggncccggn accccaattc ncccntnagg gggtccaatt acccgccccc ccccggccng
     1201 ccnttttaan ncgnccngnc ngggnaaaan ccngggggtn nccnaattta nccccctggg
     1261 nananntccc ccntttcccn ggnngggnaa aaanaaaaan ncccccccca ttccccctnc
     1321 caaagttgcc ccccggaagg gaa
//
