LOCUS       sequence-166806-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expresion of TDP-43 NLS1, 5F-L YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Elden AC, Kim HJ, Hart MP, Chen-Plotkin AS, Johnson BS, Fang X, 
            Armakola M, Geser F, Greene R, Lu MM, Padmanabhan A, Clay-Falcone D,
            McCluskey L, Elman L, Juhr D, Gruber PJ, Rub U, Auburger G, 
            Trojanowski JQ, Lee VM, Van Deerlin VM, Bonini NM, Gitler AD
  TITLE     Ataxin-2 intermediate-length polyglutamine expansions are associated
            with increased risk for ALS.
  JOURNAL   Nature. 2010 Aug 26;466(7310):1069-75. doi: 10.1038/nature09320.
  PUBMED    20740007
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-08-26- 26"; volume: "466"; issue: "7310"; 
            pages: "1069-75"
COMMENT     Sequence Label: pcDNA3.2 TDP-43 NLS1, 5F-L YFP
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
       61 ttaagctatc aacaagtttg tacaaaaaag caggcttcga aggagataac aaaatgtctg
      121 aatatattcg ggtaaccgaa gatgagaacg atgagcccat tgaaatacca tcggaagacg
      181 atgggacggt gctgctctcc acggttacag cccagtttcc aggggcgtgt gggcttcgct
      241 acaggaatcc agtgtctcag tgtatgagag gtgtccggct ggtagaagga attctgcatg
      301 ccccagatgc tggctgggga aatctggtgt atgttgtcaa ctatccaaaa gataacgcag
      361 cagcaatgga tgagacagat gcttcatcag cagtgaaagt gaaaagagca gtccagaaaa
      421 catccgattt aatagtgttg ggtctcccat ggaaaacaac cgaacaggac ctgaaagagt
      481 attttagtac ctttggagaa gttcttatgg tgcaggtcaa gaaagatctt aagactggtc
      541 attcaaaggg gttaggctta gttcgtttta cggaatatga aacacaagtg aaagtaatgt
      601 cacagcgaca tatgatagat ggacgatggt gtgactgcaa acttcctaat tctaagcaaa
      661 gccaagatga gcctttgaga agcagaaaag tgttagtggg gcgctgtaca gaggacatga
//
