LOCUS       sequence-166816-        9664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for creating transgenic zebrafish. Expresses mCherry-Cre 
            fusion protein under the fabp10 promoter and Venus under the 
            crystalin promoter in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9664)
  AUTHORS   Ni TT, Lu J, Zhu M, Maddison LA, Boyd KL, Huskey L, Ju B, Hesselson 
            D, Zhong TP, Page-McCaw PS, Stainier DY, Chen W
  TITLE     Conditional control of gene function by an invertible gene trap in 
            zebrafish.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15389-94. Epub 2012 
            Aug 20.
  PUBMED    22908272
REFERENCE   2  (bases 1 to 9664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2012-09-18"; volume: "109(38)"; pages: 
            "15389-94. Epub 2012 Aug 20"
COMMENT     Sequence Label: pHD157
FEATURES             Location/Qualifiers
     source          1..9664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     267..286
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        267..285
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3184..3204
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3205..3912
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3350..3368)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3618..3637
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3925..4956
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     polyA_signal    5254..5388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5291..5310)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5345..5364
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    5406..5453
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    5477..5684
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5673..5690
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(5708..6421,6422..6424,6425..6427))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5753..5774)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6102..6121
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(7017..7037)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7017..7035)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7068..7085)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7068..7084)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7077..7099)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(7225..7680)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7362..7383)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7574..7593
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7707..7811
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7812..7880,7881..8672)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8030..8049)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8843..9431
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9332..9351
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9585..9602
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 ttagggataa cagggtaatg cgcgcgtaat acgactcact atagggcgaa ttggagctcg
      301 gaattcctga gcatcagaat ggggaaggaa gaggatttca gtgactttga acgaggcatg
      361 gttgttgctg ccagatgggc tgctctgagt atttcagaaa ctgctgatct tcagggattt
      421 tcacgcacaa ccatctctag ggtttacaga gaatgatctg aaaagaggaa atatccagtg
      481 agcggcagtt ctgtgggtgc aaatgccttg ttgatgccag agatcagagg agaatggcca
      541 gactggttcc agctgataga aaggcaacag taactcaaat aagcactcgt tacaaccgag
      601 ctctgcagaa gagcatctct gaacacacaa cacgtccaac cttgaggcag atgggctaca
      661 gcagcagaag accacaccgg gtgccgctcc tgtcagctaa gaacaggaaa ctgaggctac
      721 aattcacaca gactcaccaa aactggacaa tatattatta gcccccctta gaatttttca
      781 tttgataata tttttcttct ggcgaaagcc tcatttgttt tatatattat agaataaaat
      841 tagtttttaa tagtttttat gccattttaa ggtcaatatt attagcccct ttaagctatt
      901 ttttttcgat agtctacaga acaaaccatc ggtatacaat gacttgccta attaccctaa
      961 cctgcctagt taccctaatt aacctagtta agcctttaaa tgtcacttta agctgtatag
     1021 aagtgtcttg aagaatatct agtctaatat tattgactgt catcatggca aagataaaat
     1081 aaatcagtta ttaaaactat tatgattaga aatgtgctga aacaatctgc tctccgataa
     1141 acagaaattg aacaaaataa acaggggggc taataaattt aaggggttaa ataattctga
     1201 ttgcaaaaaa aatgatgtct gcaaaactgt tgcaaataat ttatttgtgt tgattttaag
     1261 caaacaaatt aaatttaata aaatacaact taatctgttt gtttaaattc agccctaata
     1321 aattgtttac agccacttaa cgtaaaaaaa ttgagtaaat ccaaggaatc atctctgaat
     1381 aatttttttc agtgtatata tatatatata tattcttaca aaacaactca tttactttag
     1441 tttaattttc aggggcaaaa aactaaagta atcgacgttg cttgaataaa aagtgtaatt
     1501 aagggaatga ggtaacattt aaccatgtgt caatgcagtt taaatatgcc agttagtggt
     1561 atatgtttaa atggtaagct attcaaaact ttaaactaac ttaaccagcc ttttgttgtc
     1621 agactgaaca gactttccat ctgcattatt agagactaat ctttggctgg atgaatgatt
     1681 catctgctga tatttcagaa tagacagatt gaggctgttt ctaatatgat tatgcaacct
     1741 gagggtgatt atttgaagca aactccacag accagcaggt cattgaccgt cgtgtgttca
     1801 aacagagcag aaacatttgc aaaactggtc tgacaggaga atccagtcca gcacaacaca
     1861 tatgctgagc aaactgaatc aatcctgcag gtcaactctc gtgctttaag tttattaaga
     1921 ttattttatt tatttattat tttatttatc tatttattta tttagttgtt tatttagcaa
     1981 ttatttgttt atttatttgt ttatttattc ctgcagatca tgccttgtgc ctttttacat
     2041 ttaatttaat ttttaattta atttcctttt attttttttt atttttttat tttattttat
     2101 tttacagtct gacaaatact gaactaaaaa ccctcagatc atgtcttatg catttcattt
     2161 tattttattt cattttatat tattaatttt aatattttta ttttacagtc tgacaaatac
     2221 tgaattaaaa accatcagat catgtctcat gcatttaact taactttatt taattcaatt
     2281 aaattgtttg tttgtttgtt tccttgcatt tgtttgtttg ttttttacaa tctgacatac
     2341 tggaccgaaa aaactcagat catgtcttat gcattttatt ttattttatt ttttatttta
     2401 ttgtctattt tattttattt catcttattt tattttacag tctgacatat ggaccaataa
     2461 atctctcaga tcatgtctca tgcatttaat ttaatttttt tatttattca tttttattta
     2521 tttattttat tatttatttt attttaatat ttattttatt ttattttatt ttattttaca
     2581 gtctgacata ctggaccaat aaatctctca gatcatgtct catgcatttt acttttattt
     2641 tattagaatt agaaagatca aaggaacaac ttttaaaata ttaattctgt atcaaaatct
     2701 cttttgatac atttaattga tttaaaaaag cagttcaccc aagaaacatt tcctcacagt
     2761 cgaatggttg taaactttta tgaattactt tcacagaaaa agatttttgg aagaatattg
     2821 gaaaaaaagc agccattgac ttccatagta acaacaaaaa atactatgga agtcaatggc
     2881 tgttttttca ccattcggta tcttcattct ggagcagaat tttttgggtg atctgtccct
     2941 ttaagtcgtc aaatcctggc gcaatattcc acatgcactg acgagtcttt atttttggtc
     3001 tgctactgct gtgtgtgtga gggcatttac ctcatcttct gctggagttg atgaacggtg
     3061 ggttgttcaa acagcagcag gtcattgact gaactcctct cgatataaaa gctgcagatc
     3121 tgaagctgac cttcactttg tgttgagcgg atctaattcg atatcaagct tatcgatatg
     3181 gctccaaaga agaagcgtaa ggtaatggtg agcaagggcg aggaggataa catggccatc
     3241 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     3301 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     3361 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     3421 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacaagaa gctgtccttc
     3481 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     3541 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     3601 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     3661 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     3721 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     3781 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     3841 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     3901 gagctgtaca aggcagctag cgcaatgtcc aatttactga ccgtacacca aaatttgcct
     3961 gcattaccgg tcgatgcaac gagtgatgag gttcgcaaga acctgatgga catgttcagg
     4021 gatcgccagg cgttttctga gcatacctgg aaaatgcttc tgtccgtttg ccggtcgtgg
     4081 gcggcatggt gcaagttgaa taaccggaaa tggtttcccg cagaacctga agatgttcgc
     4141 gattatcttc tatatcttca ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg
     4201 ggccagctaa acatgcttca tcgtcggtcc gggctgccac gaccaagtga cagcaatgct
     4261 gtttcactgg ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa
     4321 caggctctag cgttcgaacg cactgatttc gaccaggttc gttcactcat ggaaaatagc
     4381 gatcgctgcc aggatatacg taatctggca tttctgggga ttgcttataa caccctgtta
     4441 cgtatagccg aaattgccag gatcagggtt aaagatatct cacgtactga cggtgggaga
     4501 atgttaatcc atattggcag aacgaaaacg ctggttagca ccgcaggtgt agagaaggca
     4561 cttagcctgg gggtaactaa actggtcgag cgatggattt ccgtctctgg tgtagctgat
     4621 gatccgaata actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc
     4681 accagccagc tatcaactcg cgccctggaa gggatttttg aagcaactca tcgattgatt
     4741 tacggcgcta aggatgactc tggtcagaga tacctggcct ggtctggaca cagtgcccgt
     4801 gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa taccggagat catgcaagct
     4861 ggtggctgga ccaatgtaaa tattgtcatg aactatatcc gtaacctgga tagtgaaaca
     4921 ggggcaatgg tgcgcctgct ggaagatggc gattaggcgg ccgccgaaat gaccgaccaa
     4981 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     5041 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     5101 ctggagttct tcgcccaccc cccggatcta agctctagat aagtaatgat cataatcagc
     5161 catatcacat ctgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     5221 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     5281 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5341 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg ggggtaccgc
     5401 gtcgagaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcgggcccg
     5461 cggtggcggc gatctctccc cagcatgcct gctattgtct tcccaatcct cccccttgct
     5521 gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat
     5581 ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac
     5641 gggggagggg caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg
     5701 ggccgcttta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     5761 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactggtagc
     5821 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     5881 agtggtcggc gagctgcacg ccgccgtcct cgatgttgtg gcggatcttg aagttggcct
     5941 tgatgccgtt cttctgcttg tcggcggtga tatagacgtt gtggctgttg tagttgtact
     6001 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     6061 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     6121 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     6181 cgtgctgctt catgtggtcg gggtagcggg cgaagcactg caggccgtag cccagggtgg
     6241 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatcagc ttcagggtca
     6301 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     6361 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     6421 tcaccatgtt gtcagacctg gtaactcctt actgtaactt attgcacctg atgaacaaaa
     6481 gatgtgaact gaacgcaagt aggaggctaa tagaggctag tacagtacaa agatccccaa
     6541 atgatgtgtg ccggccttag tggaggcaga cccctggtta tatacctgtg ctgcagaaag
     6601 aggggcttac aaatggatct ctctggaatg gcattatctc atggtgggaa cagtgttgtc
     6661 agcagaatca gcggggtgga ctctgacctg agaatgacag tctagcactg aatggctcag
     6721 acaatactct tgtggccaac tatcaccgga aaataagtgc ctgccaaaac aaaccagacc
     6781 agatgaagct acatggctgt agaacgtcag tatttaatgt gttgtggcca cttggttcac
     6841 cagatttaag agctaatttt tttaaatatc acagataatc aatcgctttt tttttttctt
     6901 gactgaaaag cttagactgg aaaattcttt gaaaaagttt gcactacaga aaataaatac
     6961 ataacatcct gcactgaatg cacactatta atgggcccgg tacccagctt ttgttccctt
     7021 tagtgagggt taattgcgcg ctagggataa cagggtaata cgcgctcact ggccgtcgtt
     7081 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     7141 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     7201 ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta aaattcgcgt
     7261 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     7321 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     7381 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     7441 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     7501 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     7561 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     7621 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     7681 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7741 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7801 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7861 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7921 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7981 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8041 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8101 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8161 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8221 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8281 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8341 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8401 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8461 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8521 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8581 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8641 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8701 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8761 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8821 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8881 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8941 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     9001 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9061 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9121 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9181 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9241 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9301 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9361 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9421 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9481 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9541 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9601 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9661 aatg
//
