LOCUS       sequence-166818-        1128 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Example plasmid containing a bidirectional promoter of pTDH3 and 
            pTEF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Jessop-Fabre MM, Jakociunas T, Stovicek V, Dai Z, Jensen MK, 
            Keasling JD, Borodina I
  TITLE     EasyClone-MarkerFree: A vector toolkit for marker-less integration 
            of genes into Saccharomyces cerevisiae via CRISPR-Cas9.
  JOURNAL   Biotechnol J. 2016 Aug;11(8):1110-7. doi: 10.1002/biot.201600147. 
            Epub 2016 Jun 23.
  PUBMED    27166612
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/biot.201600147"; journalName: "Biotechnol J"; date: 
            "2016-08"; volume: "11"; issue: "8"; pages: "1110-7"
COMMENT     Sequence Label: p1977 (pUC19-PTDH3-PTEF1)
FEATURES             Location/Qualifiers
     source          1..1128
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(13..419)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        462..1128
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     998..1021
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 ttgtaattaa aacttagatt agattgctat gctttctttc taatgagcaa gaagtaaaaa
       61 aagttgtaat agaacaagaa aaatgaaact gaaacttgag aaattgaaga ccgtttatta
      121 acttaaatat caatgggagg tcatcgaaag agaaaaaaat caaaaaaaaa aattttcaag
      181 aaaaagaaac gtgataaaaa tttttattgc ctttttcgac gaagaaaaag aaacgaggcg
      241 gtctcttttt tcttttccaa acctttagta cgggtaatta acgacaccct agaggaagaa
      301 agaggggaaa tttagtatgc tgtgcttggg tgttttgaag tggtacggcg atgcgcggag
      361 tccgagaaaa tctggaagag taaaaaagga gtagaaacat tttgaagcta tggtgtgtgc
      421 atcagtagct ataaaaaaca cgctttttca gttcgagttt atcattatca atactgccat
      481 ttcaaagaat acgtaaataa ttaatagtag tgattttcct aactttattt agtcaaaaaa
      541 ttagcctttt aattctgctg taacccgtac atgcccaaaa tagggggcgg gttacacaga
      601 atatataaca tcgtaggtgt ctgggtgaac agtttattcc tggcatccac taaatataat
      661 ggagcccgct ttttaagctg gcatccagaa aaaaaaagaa tcccagcacc aaaatattgt
      721 tttcttcacc aaccatcagt tcataggtcc attctcttag cgcaactaca gagaacaggg
      781 gcacaaacag gcaaaaaacg ggcacaacct caatggagtg atgcaacctg cctggagtaa
      841 atgatgacac aaggcaattg acccacgcat gtatctatct cattttctta caccttctat
      901 taccttctgc tctctctgat ttggaaaaag ctgaaaaaaa aggttgaaac cagttccctg
      961 aaattattcc cctacttgac taataagtat ataaagacgg taggtattga ttgtaattct
     1021 gtaaatctat ttcttaaact tcttaaattc tacttttata gttagtcttt tttttagttt
     1081 taaaacacca agaacttagt ttcgaataaa cacacataaa caaacaaa
//
