LOCUS       sequence-166820-       11478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA161.2  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11478)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM161.2
FEATURES             Location/Qualifiers
     source          1..11478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..477
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     420..440
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    645..821
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1383..1401)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1384..1401)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2040..2059
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2162..2167,2168..2184,2185..2190))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2465..2845
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2851..3402
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3412..3664
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    3750..3774
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4199..4993
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5080..5668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5822..5839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5854..5994
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5920..5939)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6080..6102
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6338..6532
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6598..7671)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8100..8729)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10030..10054
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10242..10264
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10256..10273
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10257..10273
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10298..11005)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     11184..11203
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11184..11202
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgacatctc cactgacgta
       61 agggatgacg cacaatccca cccctactcc aaaaatgtca aagatactgt ctcagaagac
      121 caaagggcta ttgagacttt tcaacaaagg gtaatttcgg ggaacctcct cggattccat
      181 tgcccagcta tctgtcacct catcgaaagg acagtagaaa aggaaggtgg ctcctacaaa
      241 tgccatcatt gcgataaagg aaaggctatc attcaagatg cctctgccga cagtggtccc
      301 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
      361 tcaaagcaag tggattgatg tgacatctcc actgacataa gggatgacgc acaaccccac
      421 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacagcc
      481 caagctttag tcactttccg aaatgcattg agttgttgtt gttatggtct aatttaaatg
      541 gcaaatatgg tctaaagaag aagaattagt cactttccga aatgcattga gaattcggta
      601 cgctgaaatc accggtctct ctctacaaat ctatctctct ctattttctc cataaataat
      661 gtgtgagtag tttcccgata agggaaatta gggttcttat agggtttcgc tcatgtgttg
      721 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
      781 tctaattcct aaaaccaaaa tccagtacta aaatccagat ctcctaaagt ccctatagat
      841 ctttgtcgtg aatataaacc agacacgaga cgactagacc tggagcccag acgccgttcg
      901 aagctagaag taccgcttag gcaggaggcc gttagggaaa agatgctaag gcagggttgg
      961 ttacgttgac tcccccgtag gtttggttta aatatgatga agtggacgga aggaaggagg
     1021 aagacaagga aggataaggt tgcaggccct gtgcaaggta agaagatgga aacttgatag
     1081 aggtacgcta ctatacttat actatacgct aagggaatga ttgtatttat accctatacc
     1141 ccctaataac cccttatcaa tttaagaaat aatccgcata agcccccgct taaaaattgg
     1201 tatcagagcc atgaataggt ctatgaccaa aactcaagag gataaaacct caccaaaata
     1261 cgaaagagtc cttaactcta aagataaaag atctttcaag atcaaaacta gttccctcac
     1321 accggtgacg gggatcgcat gcggcctcta gaccatcaca ttggagcttc agctgctcga
     1381 gttctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     1441 ccgcgttctt acgtcgacgt accaattacg ccccgccctg tcactcatcg cagtactgtt
     1501 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     1561 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     1621 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     1681 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     1741 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     1801 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     1861 gaacactatc ccatattacc agctcaccgt ctttcattgc catacggaac tccggatgag
     1921 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     1981 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     2041 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     2101 tatatccagt gatttctttc tccattttag cttccttagc tcctgaaaat ctcgataact
     2161 cattaaagct tatcgatgat aagctgtcaa acatgagaat ttagcccgcc taatgagcgg
     2221 gctttttgtt gtgatccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2281 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2341 aggcgtttcc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2401 ggatacctgt ccgcctttct taattaactc tctagactca cctaggatcc atgaattctg
     2461 agatttttca aatcagtgcg caagacgtga cgtaagtatc cgagtcagtt tttatttttc
     2521 tactaatttg gtcgtttatt tcggcgtgta ggacatggca accgggcctg aatttcgcgg
     2581 gtattctgtt tctattccaa ctttttcttg atccgcagcc attaacgact tttgaataga
     2641 tacgctgaca cgccaagcct cgctagtcaa aagtgtacca aacaacgctt tacagcaaga
     2701 acggaatgcg cgtgacgctc gcggtgacgc catttcgcct tttcagaaat ggataaatag
     2761 ccttgcttcc tattatatct tcccaaatta ccaatacatt acactagcat ctgaatttca
     2821 taaccaatct cgatacacca aatcgaatcg atgagcccag aacgacgccc ggccgacatc
     2881 cgccgtgcca ccgaggcgga catgccggcg gtctgcacca tcgtcaacca ctacatcgag
     2941 acaagcacgg tcaacttccg taccgagccg caggaaccgc aggagtggac ggacgacctc
     3001 gtccgtctgc gggagcgcta tccctggctc gtcgccgagg tggacggcga ggtcgccggc
     3061 atcgcctacg cgggcccctg gaaggcacgc aacgcctacg actggacggc cgagtcgacc
     3121 gtgtacgtct ccccccgcca ccagcggacg ggactgggct ccacgctcta cacccacctg
     3181 ctgaagtccc tggaggcaca gggcttcaag agcgtggtcg ctgtcatcgg gctgcccaac
     3241 gacccgagcg tgcgcatgca cgaggcgctc ggatatgccc cccgcggcat gctgcgggcg
     3301 gccggcttca agcacgggaa ctggcatgac gtgggtttct ggcagctgga cttcagcctg
     3361 ccggtaccgc cccgtccggt cctgcccgtc accgagattt gactgcagaa tcttggactc
     3421 ccatgttggc aaaggcaacc aaacaaacaa tgaatgatcc gctcctgcat atggggcggt
     3481 ttgagtattt caactgccat ttgggctgaa ttgaagacat gctcctgtca gaaattccgt
     3541 gatcttactc aatattcagt aatctcggcc aatatcctaa atgtgcgtgg ctttatctgt
     3601 ctttgtattg tttcatcaat tcatgtaacg tttgcttttc ttatgaattt tcaaataaat
     3661 tatcagatcc gagctcgaat taattcggcg ttaattcagt acattaaaaa cgtccgcaat
     3721 gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc
     3781 agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca
     3841 tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta
     3901 ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc
     3961 ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat caccgcggtt
     4021 tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaacaac tttgaaaaag
     4081 ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag ttcgtcttgt
     4141 tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga aataataaat
     4201 ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa
     4261 agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct
     4321 atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa
     4381 ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc actttgaacg
     4441 gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga
     4501 gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct
     4561 ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca gccgcttagc
     4621 cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga
     4681 agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga
     4741 agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg
     4801 caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat
     4861 tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg tcgagctatt
     4921 ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga
     4981 tgaattgttt tagtacctag aatgcatgac caaaatccct taacgtgagt tttcgttcca
     5041 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5101 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     5161 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     5221 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     5281 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     5341 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5401 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5461 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5521 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5581 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5641 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5701 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5761 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     5821 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     5881 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     5941 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     6001 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     6061 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     6121 aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc
     6181 ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc
     6241 cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc
     6301 tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt
     6361 aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat
     6421 atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg
     6481 gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag
     6541 accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta
     6601 ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc
     6661 agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt
     6721 gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt
     6781 cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa
     6841 acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt
     6901 cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg
     6961 ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac
     7021 cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt
     7081 taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca
     7141 gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc
     7201 ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta
     7261 atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag
     7321 ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac
     7381 gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc
     7441 tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg
     7501 ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc
     7561 tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc
     7621 acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc
     7681 tcgggcttgg gggttccagt gccattgcag ggccggcagg caacccagcc gcttacgcct
     7741 ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct
     7801 tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct
     7861 catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg
     7921 gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg
     7981 cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct
     8041 cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact
     8101 caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc
     8161 tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc
     8221 tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca
     8281 ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca
     8341 tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg
     8401 taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc
     8461 aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc
     8521 acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc
     8581 acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc
     8641 ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct
     8701 ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc
     8761 atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc
     8821 tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct
     8881 tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg
     8941 gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg
     9001 gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc
     9061 ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc
     9121 ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac
     9181 gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc
     9241 gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt
     9301 ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat
     9361 gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc
     9421 tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc
     9481 gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag
     9541 gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga
     9601 aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc
     9661 gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta
     9721 atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa
     9781 acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc
     9841 gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg
     9901 ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg
     9961 acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt
    10021 ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg
    10081 ggaaacgaca atctgatcca agctcaagct gctctagcat tcgccattca ggctgcgcaa
    10141 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    10201 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    10261 aacgacggcc agtgccaagc ttgcatgcct gcaggtcctg ctgagcctcg acatgttgtc
    10321 gcaaaattcg ccctggaccc gcccaacgat ttgtcgtcac tgtcaaggtt tgacctgcac
    10381 ttcatttggg gcccacatac accaaaaaaa tgctgcataa ttctcggggc agcaagtcgg
    10441 ttacccggcc gccgtgctgg accgggttga atggtgcccg taactttcgg tagagcggac
    10501 ggccaatact caacttcaag gaatctcacc catgcgcgcc ggcggggaac cggagttccc
    10561 ttcagtgagc gttattagtt cgccgctcgg tgtgtcgtag atactagccc ctggggcact
    10621 tttgaaattt gaataagatt tatgtaatca gtcttttagg tttgaccggt tctgccgctt
    10681 tttttaaaat tggatttgta ataataaaac gcaattgttt gttattgtgg cgctctatca
    10741 tagatgtcgc tataaaccta ttcagcacaa tatattgttt tcattttaat attgtacata
    10801 taagtagtag ggtacaatca gtaaattgaa cggagaatat tattcataaa aatacgatag
    10861 taacgggtga tatattcatt agaatgaacc gaaaccggcg gtaaggatct gagctacaca
    10921 tgctcaggtt ttttacaacg tgcacaacag aattgaaagc aaatatcatg cgatcatagg
    10981 cgtctcgcat atctcattaa agcaggactc tagggactag tcccgggtct taattaagtt
    11041 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag gagatccttt ggatcacaaa
    11101 aaaaaagccc gctcattagg cgggttaaat tcccgggtac tgcagccgat tcattaatgc
    11161 aggttaacct ggcttatcga aattaatacg actcactata gggagaccgg cagatctgat
    11221 ccctactcca aaaatgtcaa agatacagtc tcagaagacc aaagggctat tgagactttt
    11281 caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
    11341 atcgaaagga cagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
    11401 aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg
    11461 aggagcatcg tgggaaaa
//
