LOCUS       sequence-166823-       11477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  target  miRNA163  for destruction in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Tang G, Yan J, Gu Y, Qiao M, Fan R, Mao Y, Tang X
  TITLE     Construction of short tandem target mimic (STTM) to block the 
            functions of plant and animal microRNAs.
  JOURNAL   Methods. 2012 Oct;58(2):118-25. doi: 10.1016/j.ymeth.2012.10.006. 
            Epub 2012 Oct 23.
  PUBMED    23098881
REFERENCE   2  (bases 1 to 11477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymeth.2012.10.006"; journalName: "Methods"; date: 
            "2012-10"; volume: "58"; issue: "2"; pages: "118-25"
COMMENT     Sequence Label: pFGC5941-PacI-ath-STTM163
FEATURES             Location/Qualifiers
     source          1..11477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    513..689
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1251..1269)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1252..1269)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1908..1927
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2030..2035,2036..2052,2053..2058))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     promoter        2333..2713
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             2719..3270
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      3280..3532
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    3618..3642
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4067..4861
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4948..5536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5690..5707
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5722..5862
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5788..5807)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5948..5970
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6206..6400
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6466..7539)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7968..8597)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9898..9922
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10110..10132
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10124..10141
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10125..10141
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10166..10873)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     11052..11071
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11052..11070
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacctc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gacatctcca ctgacataag ggatgacgca caaccccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttatcg
      361 aagttccaag ctatcctctt caagttgttg ttgttatggt ctaatttaaa tatggtctaa
      421 agaagaagaa tatcgaagtt ccaagctatc ctcttcaaga attcggtacg ctgaaatcac
      481 cagtctctct ctacaaatct atctctctct attttctcca taaataatgt gtgagtagtt
      541 tcccgataag ggaaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa
      601 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
      661 aaccaaaatc cagtactaaa atccagatct cctaaagtcc ctatagatct ttgtcgtgaa
      721 tataaaccag acacgagacg actagacctg gagcccagac gccgttcgaa gctagaagta
      781 ccgcttaggc aggaggccgt tagggaaaag atgctaaggc agggttggtt acgttgactc
      841 ccccgtaggt ttggtttaaa tatgatgaag tggacggaag gaaggaggaa gacaaggaag
      901 gataaggttg caggccctgt gcaaggtaag aagatggaaa tttgatagag gtacgctact
      961 atacttatac tatacgctaa gggaatgatt gtatttatac cctatacccc ctaataaccc
     1021 cttatcaatt taagaaataa tccgcataag cccccgctta aaaattggta tcagagccat
     1081 gaataggtct atgaccaaaa ctcaagagga taaaacctca ccaaaatacg aaagagtcct
     1141 taactctaaa gataaaagat ctttcaagat caaaactagt tccctcacac cggtgacggg
     1201 gatcgcatgc gacctctaga ccatcacatt ggagcttcag ctgctcgagt tctatagtgt
     1261 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttcttac
     1321 gtcgacgtac caattacgcc ccgccctgtc actcatcgca gtactgttgt aattcattaa
     1381 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     1441 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     1501 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
     1561 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     1621 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     1681 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     1741 atattaccag ctcaccgtct ttcattgcca tacggaactc cggatgagca ttcatcaggc
     1801 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1861 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1921 atgcctcaaa atgttcttta cgatgccatt ggggtatatc aacggtggta tatccagtga
     1981 tttctttctc cattttagct tccttagctc ctgaaaatct cgataactca ttaaagctta
     2041 tcgatgataa gctgtcaaac atgagaattt agcccgccta atgagcgggc tttttgttgt
     2101 gatccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2161 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2221 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2281 gcctttctta attaactctc tagactcacc taggatccat gaattctgag atttttcaaa
     2341 tcagtgcgca agacgtgacg taagtatccg agtcagtttt tatttttcta ctaatttggt
     2401 cgtttatttc ggcgtgtagg acatggcaac cgggcctgaa tttcgcgggt attctgtttc
     2461 tattccaact ttttcttgat ccgcagccat taacgacttt tgaatagata cgctgacacg
     2521 ccaagcctcg ctagtcaaaa gtgtaccaaa caacgcttta cagcaagaac ggaatgcgcg
     2581 tgacgctcgc ggtgacgcca tttcgccttt tcagaaatgg ataaatagcc ttgcttccta
     2641 ttatatcttc ccaaattacc aatacattac actagcatct gaatttcata accaatctcg
     2701 atacaccaaa tcgaatcgat gagcccagaa cgacgcccgg ccgacatccg ccgtgccacc
     2761 gaggcggaca tgccggcggt ctgcaccatc gtcaaccact acatcgagac aagcacggtc
     2821 aacttccgta ccgagccgca ggaaccgcag gagtggacgg acgacctcgt ccgtctgcgg
     2881 gagcgctatc cctggctcgt cgccgaggtg gacggcgagg tcgccggcat cgcctacgcg
     2941 ggcccctgga aggcacgcaa cgcctacgac tggacggccg agtcgaccgt gtacgtctcc
     3001 ccccgccacc agcggacggg actgggctcc acgctctaca cccacctgct gaagtccctg
     3061 gaggcacagg gcttcaagag cgtggtcgct gtcatcgggc tgcccaacga cccgagcgtg
     3121 cgcatgcacg aggcgctcgg atatgccccc cgcggcatgc tgcgggcggc cggcttcaag
     3181 cacgggaact ggcatgacgt gggtttctgg cagctggact tcagcctgcc ggtaccgccc
     3241 cgtccggtcc tgcccgtcac cgagatttga ctgcagaatc ttggactccc atgttggcaa
     3301 aggcaaccaa acaaacaatg aatgatccgc tcctgcatat ggggcggttt gagtatttca
     3361 actgccattt gggctgaatt gaagacatgc tcctgtcaga aattccgtga tcttactcaa
     3421 tattcagtaa tctcggccaa tatcctaaat gtgcgtggct ttatctgtct ttgtattgtt
     3481 tcatcaattc atgtaacgtt tgcttttctt atgaattttc aaataaatta tcagatccga
     3541 gctcgaatta attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt
     3601 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
     3661 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
     3721 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
     3781 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
     3841 gttgattgta acgatgacag agcgttgctg cctgtgatca ccgcggtttc aaaatcggct
     3901 ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt
     3961 atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt
     4021 cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag
     4081 aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg
     4141 aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat
     4201 gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct
     4261 atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg
     4321 gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga
     4381 acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat
     4441 cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta
     4501 cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt
     4561 taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt
     4621 cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg
     4681 ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt
     4741 ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact
     4801 ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta
     4861 gtacctagaa tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     5041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     5101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     5161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     5221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     5281 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5341 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5461 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5521 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5581 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5641 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     5701 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     5761 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     5821 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     5881 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     5941 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     6001 cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc
     6061 tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg
     6121 acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc
     6181 ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat
     6241 aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta
     6301 catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc
     6361 aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc
     6421 tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga
     6481 tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc
     6541 ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa
     6601 acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca
     6661 tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc
     6721 gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt
     6781 gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc
     6841 gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg
     6901 gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca
     6961 ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac
     7021 gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg
     7081 gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact
     7141 ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat
     7201 cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct
     7261 gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc
     7321 gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt
     7381 gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg
     7441 ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc
     7501 gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg
     7561 gttccagtgc cattgcaggg ccggcaggca acccagccgc ttacgcctgg ccaaccgccc
     7621 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
     7681 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
     7741 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
     7801 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
     7861 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
     7921 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
     7981 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
     8041 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
     8101 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
     8161 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
     8221 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
     8281 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
     8341 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
     8401 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
     8461 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
     8521 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
     8581 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
     8641 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
     8701 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
     8761 aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt
     8821 acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc
     8881 cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc
     8941 gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt
     9001 cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc
     9061 agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg
     9121 tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg
     9181 cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac
     9241 ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg
     9301 caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg
     9361 ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat
     9421 cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt
     9481 gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac
     9541 gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt
     9601 ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa
     9661 gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga
     9721 cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt
     9781 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
     9841 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt
     9901 tacccgccaa tatatcctgt caaacactga tagtttaaac tgaaggcggg aaacgacaat
     9961 ctgatccaag ctcaagctgc tctagcattc gccattcagg ctgcgcaact gttgggaagg
    10021 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    10081 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    10141 tgccaagctt gcatgcctgc aggtcctgct gagcctcgac atgttgtcgc aaaattcgcc
    10201 ctggacccgc ccaacgattt gtcgtcactg tcaaggtttg acctgcactt catttggggc
    10261 ccacatacac caaaaaaatg ctgcataatt ctcggggcag caagtcggtt acccggccgc
    10321 cgtgctggac cgggttgaat ggtgcccgta actttcggta gagcggacgg ccaatactca
    10381 acttcaagga atctcaccca tgcgcgccgg cggggaaccg gagttccctt cagtgagcgt
    10441 tattagttcg ccgctcggtg tgtcgtagat actagcccct ggggcacttt tgaaatttga
    10501 ataagattta tgtaatcagt cttttaggtt tgaccggttc tgccgctttt tttaaaattg
    10561 gatttgtaat aataaaacgc aattgtttgt tattgtggcg ctctatcata gatgtcgcta
    10621 taaacctatt cagcacaata tattgttttc attttaatat tgtacatata agtagtaggg
    10681 tacaatcagt aaattgaacg gagaatatta ttcataaaaa tacgatagta acgggtgata
    10741 tattcattag aatgaaccga aaccggcggt aaggatctga gctacacatg ctcaggtttt
    10801 ttacaacgtg cacaacagaa ttgaaagcaa atatcatgcg atcataggcg tctcgcatat
    10861 ctcattaaag caggactcta gggactagtc ccgggtctta attaagtttg caagcagcag
    10921 attacgcgca gaaaaaaagg atctcaagga gatcctttgg atcacaaaaa aaaagcccgc
    10981 tcattaggcg ggttaaattc ccgggtactg cagccgattc attaatgcag gttaacctgg
    11041 cttatcgaaa ttaatacgac tcactatagg gagaccggca gatctgatcc ctactccaaa
    11101 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
    11161 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
    11221 gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
    11281 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
    11341 ggaaaagaag gcgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
    11401 gacgtaaggg atgacgcaca atcccacccc tactccaaaa atgtcaaaga tactgtctca
    11461 gaagaccaag gggctat
//
