LOCUS       sequence-166827-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DONOR vector for Gateway cloning of EWSR1  c1655t Stop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Couthouis J, Hart MP, Erion R, King OD, Diaz Z, Nakaya T, Ibrahim F,
            Kim HJ, Mojsilovic-Petrovic J, Panossian S, Kim CE, Frackelton EC, 
            Solski JA, Williams KL, Clay-Falcone D, Elman L, McCluskey L, Greene
            R, Hakonarson H, Kalb RG, Lee VM, Trojanowski JQ, Nicholson GA, 
            Blair IP, Bonini NM, Van Deerlin VM, Mourelatos Z, Shorter J, Gitler
            AD
  TITLE     Evaluating the role of the FUS/TLS-related gene EWSR1 in amyotrophic
            lateral sclerosis.
  JOURNAL   Hum Mol Genet. 2012 Jul 1;21(13):2899-911. doi: 10.1093/hmg/dds116. 
            Epub 2012 Mar 27.
  PUBMED    22454397
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/dds116"; journalName: "Hum Mol Genet"; date: 
            "2012-07-1- 1"; volume: "21"; issue: "13"; pages: "2899-911"
COMMENT     Sequence Label: pDONR221-EWSR1 c1655t Stop
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
       61 ttataatgcc aactttgtac aaaaaagcag gcttcgaagg agataacaaa atggcgtcca
      121 cggattacag tacctatagc caagctgcag cgcagcaggg ctacagtgct tacaccgccc
      181 agcccactca aggatatgca cagaccaccc aggcatatgg gcaacaaagc tatggaacct
      241 atggacagcc cactgatgtc agctataccc aggctcagac cactgcaacc tatgggcaga
      301 ccgcctatgc aacttcttat ggacagcctc ccactggtta tactactcca actgcccccc
      361 aggcatacag ccagcctgtc caggggtatg gcactggtgc ttatgatacc accactgcta
      421 cagtcaccac cacccaggcc tcctatgcag ctcagtctgc atatggcact cagcctgctt
      481 atccagccta tgggcagcag ccagcagcca ctgcacctac aagaccgcag gatggaaaca
      541 agcccactga gactagtcaa cctcaatcta gcacaggggg ttacaaccag cccagcctag
      601 gatatggaca gagtaactac agttatcccc aggtacctgg gagctacccc atgcagccag
      661 tcactgcacc tccatcctac cctcctacca gctattcctc tacacagccg actagttatg
      721 atcagagcag ttactctcag cagaacacct atgggcaacc gagcagctat ggacagcaga
      781 gtagctatgg tcaacaaagc agctatgggc agcagcctcc cactagttac ccaccccaaa
      841 ctggatccta cagccaagct ccaagtcaat atagccaaca gagcagcagc tacgggcagc
      901 agagttcatt
//
