LOCUS       sequence-166828-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DONOR vector for Gateway cloning of EWSR1  c1655t Stop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Couthouis J, Hart MP, Erion R, King OD, Diaz Z, Nakaya T, Ibrahim F,
            Kim HJ, Mojsilovic-Petrovic J, Panossian S, Kim CE, Frackelton EC, 
            Solski JA, Williams KL, Clay-Falcone D, Elman L, McCluskey L, Greene
            R, Hakonarson H, Kalb RG, Lee VM, Trojanowski JQ, Nicholson GA, 
            Blair IP, Bonini NM, Van Deerlin VM, Mourelatos Z, Shorter J, Gitler
            AD
  TITLE     Evaluating the role of the FUS/TLS-related gene EWSR1 in amyotrophic
            lateral sclerosis.
  JOURNAL   Hum Mol Genet. 2012 Jul 1;21(13):2899-911. doi: 10.1093/hmg/dds116. 
            Epub 2012 Mar 27.
  PUBMED    22454397
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/dds116"; journalName: "Hum Mol Genet"; date: 
            "2012-07-1- 1"; volume: "21"; issue: "13"; pages: "2899-911"
COMMENT     Sequence Label: pDONR221-EWSR1 c1655t Stop
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..95
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt
       61 tcttataatg ccaactttgt acaagaaagc tgggtcctag tagggccgat ctctgcgctc
      121 ctgacggtgc tcgcctttat ccatttttcc aggtcctcca cgtcctcctc ttcttcctcc
      181 catctgttcc atcaaaggtc cagggggccc cccagggcca cctcgtcttc ctccaccaaa
      241 gccacctcgg tccatgcccc ggccaccacg gaagccacct ctgtctccac cacggccacc
      301 tctgaacatt ccaccgggac caccacgatc catgaggcca cctcttcctc cccgcatgcc
      361 accagggcca cctctgccac gatcaccacc cgggggcgga aagggtggca ggaggaagcc
      421 ttcaggcttt ggggccttac actggttgca ctctgttctc caggcgaagt tctggtttcc
      481 acaacccgga ttgggacact gccagtctcc agctcggtgc tggacgtttc ctcctccaga
      541 ggggttccct cgggaacccc ggggtcctct tggagggaag cctcctctat ctcctccacg
      601 gcctcccatg cgacccatgg gtcccccagg acctcctggg cctcctggac ctccacggag
      661 tggtggtggc atgcctctgc cctcacgggg tggcagacca ccccgcatac tgttcattgg
      721 aggcttcttc cgagcaaggg agactttaag tttgctccct tgaaaatctt tcccatcaaa
      781 ccattccacg gcagccttgg cagtgggtgg gtcttcatag gacactgtgg catcgccttt
      841 gggctttcct gtttccttgt ccaggtagat gtggatcatg ggttgcccag ttctcttgtt
//
