LOCUS       sequence-166829-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DONOR vector for Gateway cloning of EWSR1 c1655t No Stop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Couthouis J, Hart MP, Erion R, King OD, Diaz Z, Nakaya T, Ibrahim F,
            Kim HJ, Mojsilovic-Petrovic J, Panossian S, Kim CE, Frackelton EC, 
            Solski JA, Williams KL, Clay-Falcone D, Elman L, McCluskey L, Greene
            R, Hakonarson H, Kalb RG, Lee VM, Trojanowski JQ, Nicholson GA, 
            Blair IP, Bonini NM, Van Deerlin VM, Mourelatos Z, Shorter J, Gitler
            AD
  TITLE     Evaluating the role of the FUS/TLS-related gene EWSR1 in amyotrophic
            lateral sclerosis.
  JOURNAL   Hum Mol Genet. 2012 Jul 1;21(13):2899-911. doi: 10.1093/hmg/dds116. 
            Epub 2012 Mar 27.
  PUBMED    22454397
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/dds116"; journalName: "Hum Mol Genet"; date: 
            "2012-07-1- 1"; volume: "21"; issue: "13"; pages: "2899-911"
COMMENT     Sequence Label: pDONR221-EWSR1 c1655t No Stop
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
       61 tttttataat gccaactttg tacaaaaaag caggcttcga aggagataac aaaatggcgt
      121 ccacggatta cagtacctat agccaagctg cagcgcagca gggctacagt gcttacaccg
      181 cccagcccac tcaaggatat gcacagacca cccaggcata tgggcaacaa agctatggaa
      241 cctatggaca gcccactgat gtcagctata cccaggctca gaccactgca acctatgggc
      301 agaccgccta tgcaacttct tatggacagc ctcccactgg ttatactact ccaactgccc
      361 cccaggcata cagccagcct gtccaggggt atggcactgg tgcttatgat accaccactg
      421 ctacagtcac caccacccag gcctcctatg cagctcagtc tgcatatggc actcagcctg
      481 cttatccagc ctatgggcag cagccagcag ccactgcacc tacaagaccg caggatggaa
      541 acaagcccac tgagactagt caacctcaat ctagcacagg gggttacaac cagcccagcc
      601 taggatatgg acagagtaac tacagttatc cccaggtacc tgggagctac cccatgcagc
      661 cagtcactgc acctccatcc taccctccta ccagctattc ctctacacag ccgactagtt
      721 atgatcagag cagttactct cagcagaaca cctatgggca accgagcagc tatg
//
